FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448234478
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG7578490.1690742317238702TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1828100.079.345171
TCGGAAG1984550.072.946622
CGGAAGA2198800.065.737393
AGAGCAC4685900.031.1638347
AGCACAC5016350.029.122179
GAGCACA5091900.028.7882448
GAAGAGC5222300.028.1572135
TATGCCG1405200.020.66716445-49
GCGCATC1422400.020.13769135-39
TAATGCG1485200.019.76410330-34
AAGAGCA7673250.019.437336
CGTATGC1514300.018.92734745-49
AATGCGC1531300.018.86331230-34
CTCGTAT1369350.018.58821340-44
TGCGCAT1577800.018.2167835-39
ATGCGCA1599950.018.04069535-39
TCTCGTA1405050.018.01273240-44
ATGCCGT1671350.017.27285445-49
GGAAGAG8748550.017.210434
TGCCGTC1698200.016.7206145-49
Produced by FastQC (version 0.11.5)