FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443176913
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG8647990.19513629312183958TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2117050.069.35481
TCGGAAG2273350.064.6483462
CGGAAGA2496550.058.87753
AGAGCAC5010650.029.7632797
AGCACAC5312550.028.0104249
GAGCACA5440000.027.4741788
GAAGAGC5547250.026.9839485
GATCGGA938950.021.7249261
TATGCCG1566850.021.41722345-49
CGTATGC1682000.019.78462245-49
CTCGTAT1602950.019.7722140-44
TCTCGTA1655250.019.1667540-44
AAGAGCA7986250.019.0824996
GTCACGA1811200.018.56446625-29
ATGCCGT1840350.018.33991645-49
GCCGTCT1911850.017.43132850-54
ACGAGAT1964350.017.20373230-34
ATCTCGT1890250.016.88438640-44
GGAAGAG9059500.016.8681644
AGTCACG2048100.016.51270525-29
Produced by FastQC (version 0.11.5)