FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443176913
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC9361740.21124159958215155Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2265100.064.826999
GAGCGTC2630650.055.9436578
ATCGGAA2796650.053.1073531
TCGGAAG2966950.050.068762
AGAGCGT2976500.049.581077
CGGAAGA3173950.046.7694243
AAGAGCG3379400.043.910876
GAAGAGC6239050.024.2324715
CGCCGTA1655600.020.10777355-59
TCGCCGT1811850.018.41773455-59
GCCGTAT1910750.017.55852155-59
GTCGCCG1934400.017.21643855-59
GTACGTC2038100.016.25651630-34
TGGTCGC2086850.016.1612450-54
CGTATCA2099450.015.90571460-64
TACGTCC2119950.015.8394930-34
GGAAGAG9892750.015.6228244
GTGGTCG2212300.015.32279650-54
GCGTCGT2208350.015.15635310-14
GTGTACG2248000.014.92055225-29
Produced by FastQC (version 0.11.5)