FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419465248
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG24504620.5841871315165541TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4330700.095.0876541
TCGGAAG4499150.091.606032
CGGAAGA4672400.088.1219943
AGAGCAC7009750.058.957687
AGCACAC7268350.056.7337539
GAGCACA7362850.056.123718
GAAGAGC7586000.054.574665
AAGAGCA9951650.041.8723876
GGAAGAG10966850.038.139294
TATGCCG3341600.025.05339445-49
CTCGTAT3287400.023.99517440-44
CGTATGC3467450.023.90288745-49
TCTCGTA3321350.023.73768240-44
ATGCCGT3599450.023.24055145-49
TTACTCG3570300.023.02342630-34
TGCCGTC3605950.022.83004845-49
TACTCGA3624050.022.7846135-39
GCCGTCT3627150.022.75535650-54
TCGTATG3461600.022.47150640-44
ATCTCGT3547400.022.23526840-44
Produced by FastQC (version 0.11.5)