FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419465248
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC26463730.6308920733285633Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4088050.0100.8170859
GAGCGTC4432550.092.9619148
ATCGGAA4720750.088.730331
AGAGCGT4806250.085.98277
TCGGAAG4875550.085.6287462
CGGAAGA5062800.082.282023
AAGAGCG5228350.079.4652946
GAAGAGC7883600.053.0944145
GGAAGAG11304500.037.4101944
CGCCGTA3249200.025.68130755-59
TCGCCGT3375400.024.73358355-59
GCCGTAT3467450.024.11921155-59
GTCGCCG3501550.023.8280255-59
CCGTATC3585600.022.97957455-59
TGGTCGC3660800.022.95869850-54
CGTATCA3666900.022.69587360-64
GGTCGCC3672950.022.58001150-54
GTGGTCG3782600.022.28151550-54
GCGTCGT4003400.020.84290510-14
TCTCGGT4084600.020.73110245-49
Produced by FastQC (version 0.11.5)