FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485181560
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1338600.030.4247029
GAGCGTC1754450.023.3826968
ATCGGAA1991350.020.8380431
AGAGCGT2067600.019.9850627
TCGGAAG2151500.019.4568422
CGGAAGA2346250.017.8153573
AAGAGCG2488400.016.8095366
CGCCGTA695850.012.03688355-59
TCGCCGT851850.09.96701955-59
GCCGTAT957600.09.05710955-59
GTCGCCG981800.08.66846955-59
TGGTCGC1098400.07.913126550-54
GAAGAGC5754100.07.8452325
CCGTATC1105850.07.753761355-59
CGTATCA1180400.07.4921160-64
GTGGTCG1219000.07.27297750-54
GGTCGCC1175800.07.265219750-54
CAGTACG1197400.07.048187730-34
AGTACGT1316050.06.63083735-39
GCGTCGT1279450.06.579410-14
Produced by FastQC (version 0.11.5)