FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462647324
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG15249660.32961738259184226TruSeq Adapter, Index 8 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4847700.059.0424581
TCGGAAG4840700.058.9941332
CGGAAGA5101800.055.9151953
AGAGCAC7151450.040.0638287
AGCACAC7333650.038.9743089
GAGCACA7533700.038.0762638
GAAGAGC8091700.035.5778735
AAGAGCA10550550.027.4676826
GGAAGAG11379800.025.6047614
TATGCCG2964100.019.47920645-49
TCTCGCG3092850.018.6780430-34
CGTATGC3066150.018.62378945-49
CGCGCAT2960050.018.61334235-39
CTCGTAT2846050.017.93140240-44
TCGCGCA3179750.017.8242535-39
TCTCGTA2822900.017.5900440-44
ATGCCGT3334450.017.28351845-49
TCGTATG2928550.017.14902940-44
GCGCATC3284900.016.91077235-39
ACTCTCG3441650.016.82467830-34
Produced by FastQC (version 0.11.5)