FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460346682
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG17005910.3694152834145984TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA6165350.049.2185941
TCGGAAG6209850.048.8376122
CGGAAGA6298650.048.140673
AGAGCAC8514200.035.798557
AGCACAC8699950.035.0367289
GAGCACA8886150.034.3180668
GAAGAGC9261050.033.0462235
AAGAGCA11673900.026.4003876
GGAAGAG12949550.023.9308174
TATGCCG3046950.020.01214245-49
CGTATGC3214800.018.8216245-49
ATGCCGT3310200.018.35932545-49
CTCGTAT3013000.018.28987740-44
GCCGTCT3332000.017.97241250-54
TGCCGTC3337450.017.96835145-49
TCTCGTA3100100.017.74561140-44
TCGTATG3136300.017.35497540-44
CGTCTTC3583450.016.95244250-54
TACTCGA3628200.016.63706435-39
CCGTCTT3657500.016.61992550-54
Produced by FastQC (version 0.11.5)