FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004973714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34788559
Sequences flagged as poor quality0
Sequence length35-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA25450.016.3674814
TATTGCG37550.015.84519542
CGTATCG26250.015.6989443
CCCGTAT31750.015.2251351
TTGCGCC51150.013.11077444
GGTACTA47200.012.83617331
CCGTATC34400.012.7571212
CGGTACT54900.011.23868430
TAGCGGG49650.011.21674535
TAAGCGT63750.010.971331
CCGGTCG34800.010.8817223
GCGACAG60550.010.850450512
CGATTTC58500.010.85040119
TGCATTG99050.010.668056545
TGCGGTA62250.010.59146428
CTTCGAT63400.010.519091
CTAAGCG66950.010.48016230
AAAGCCG50400.010.47427526
TCGATTG30900.010.45218125
CCGGTAA19700.010.4513581
Produced by FastQC (version 0.11.5)