FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004973717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30331219
Sequences flagged as poor quality0
Sequence length35-51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATG507100.16718747769418696No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN381770.12586701510414072No Hit
CTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATTG314070.10354677799134944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGG6100.029.0209731
ATTCGGT8700.027.3024331
CGTCTAG4650.026.022891
CGCGTAG3550.023.9866711
CGATCGT851.9730032E-723.7267531
GTATCGA38050.022.3036294
CGCGTAT855.2199757E-621.0904461
AAAGCCG43850.020.57331526
CGCCAGT12850.020.4031621
CGCGATT1101.04442734E-720.3714541
TTGGACT45200.020.27361745
CCCGTAT43450.019.7525751
TCGGTTC14550.018.9865933
TTGCGCC44500.018.6587744
CGTGGCA8050.018.6506351
CGACCGT9300.018.5533581
AGCCGGT50050.018.337128
CGGGTAT8650.018.1341271
AAAAGCC50050.017.93564225
CGCGGAT2003.6379788E-1217.926881
Produced by FastQC (version 0.11.5)