FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004973723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1534694
Sequences flagged as poor quality0
Sequence length44-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1276188.315533910994635No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC955736.227495513763656No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC419482.7333136117037014RNA PCR Primer, Index 16 (100% over 51bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA374422.4397045925767613No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA343692.2394692362125608No Hit
CCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGT237821.5496248763597171RNA PCR Primer, Index 16 (100% over 46bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCC221841.4454998846675624RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC196551.2807113339857978RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC184161.1999786276612796RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC182711.190530490117248No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC166021.0817791690069811RNA PCR Primer, Index 1 (100% over 33bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCA159741.040858959505934No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC137970.899006577206922No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC134650.8773736002095531No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC132520.8634946119552172No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC128190.8352805184616608No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC125670.8188603070058266RNA PCR Primer, Index 5 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110570.7204693574093598No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTGGAATTCTCGGGTGC107590.7010518057671431No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAAGG103890.6769427651375454Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC103270.6729028718428559No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC102030.6648230852534772No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC100930.6576555326338671RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA96350.6278124499085811No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC90650.5906714954251466RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCA86200.5616754871003601No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA85360.5562020832817487No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA84540.5508589986016756No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC84020.5474707009996781No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC82350.5365890529317245No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTGGAATTCTCGGGTGCC79760.519712724491006No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC75570.49241086496721825No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCC70190.4573550167003976RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCC68580.4468643260480591RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC59930.39050129863021554No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC58260.37961965056226193No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC57890.37720874649930214No Hit
CCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTAT53570.34905981257501495RNA PCR Primer, Index 16 (100% over 48bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC50780.3308802927489128RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT49000.31928188941899815RNA PCR Primer, Index 1 (100% over 30bp)
CCCCGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCT47960.3125052942150031RNA PCR Primer, Index 16 (100% over 43bp)
GCATGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAGG46160.30077657174655015Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATGGAATTCTCGGGTGCCA36780.23965689577205618No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC35750.2329454601373303RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGAATTCTCGGGTGCC35550.23164226875194666No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGC34720.22623402450260444No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCC34120.22232445034645346RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGC32770.21352790849511366No Hit
GTTCTTGTTGTTGAAATACAACGATGGTTTTTCATATCATTGGTCGTGGTT31910.2079241855379639No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC31430.20479652621304312RNA PCR Primer, Index 1 (100% over 27bp)
CCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATC30790.20062631377981538RNA PCR Primer, Index 16 (100% over 42bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCCG28450.1853789745708265RNA PCR Primer, Index 16 (100% over 51bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCC27070.1763869540116792RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC26110.17013163536183762RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTGGAATTCTCGGGTGCC26020.16954519923841496No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT25950.16908908225353067RNA PCR Primer, Index 1 (100% over 30bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC25660.16719945474472436No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC24840.1618563700646513No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCC23910.1557965301226173No Hit
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC23300.1518217963971971RNA PCR Primer, Index 1 (100% over 31bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC22790.14849865836446874No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT22160.1443936055005102RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGTGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCA21440.139702116513129No Hit
TCTGAAGGTCGTGAGTTCGTTCCTCACACGGGGCACCATGGAATTCTCGGG20570.13403323398671005No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC20480.1334467978632874No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA20380.13279520217059557No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG20240.131882968200827Illumina Small RNA Adapter 2 (100% over 21bp)
NCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA20090.13090557466178926No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT19750.12869014930663703RNA PCR Primer, Index 1 (100% over 30bp)
CCCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCAT19350.1260837665358697RNA PCR Primer, Index 16 (100% over 41bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGG19310.12582312825879297Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC18850.12282578807241054RNA PCR Primer, Index 2 (100% over 34bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGCCA18680.12171807539483441No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGCC18260.11898137348552872No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA18250.11891621391625952No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATGGAATTCTCGGGTGCC18230.11878589477772117No Hit
GCATTGGTGGTTCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC18070.11774334166941423RNA PCR Primer, Index 1 (100% over 33bp)
TCTGAAGGTCGTGAGTTCGTTCCTCACACGGGGCACCTGGAATTCTCGGGT17670.11513695889864689No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC17620.11481116105230098RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA17570.11448536320595507No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC17380.1132473313898406RNA PCR Primer, Index 1 (100% over 24bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCC17150.11174866129664937RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCC16310.10627525747803797RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC15990.1041901512614241RNA PCR Primer, Index 1 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA15970.10405983212288573No Hit
NTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC15940.10386435341507819No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCC15860.10334307686092471RNA PCR Primer, Index 1 (100% over 27bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTCTGGAATTCTCGGGTGCC15540.10125797064431084No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTGGAATTCTCGGGTGC15480.10086701322869576No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA15470.10080185365942657No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)