FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004973727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34424306
Sequences flagged as poor quality0
Sequence length35-51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN357070.10372612885790639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA26500.016.4682394
CCCGTAT32200.015.4723561
ACTATCG17850.012.56927945
CGTATCG38200.012.2403233
CCGTATC43600.011.3371382
TCGAAGG68150.010.357117
ATCGAAG68600.09.9970476
GTCGCTA21550.09.92139427
GGTATGT67700.09.60101745
CGCGATT4102.3403118E-89.3051721
CGCGTAA12800.09.2923431
GTAGCGT21200.09.032495518
TAGCGTG22200.08.92674119
CGCGTTA25000.08.91058134
CGCGTTT18550.08.8315771
TATCGAA56400.08.6062585
TCGCGTT27400.08.53631133
CCGGTAT25750.08.3666831
CGCGTAG7101.8189894E-128.2181561
TTACGGT18500.08.18698230
Produced by FastQC (version 0.11.5)