FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004973735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35469101
Sequences flagged as poor quality0
Sequence length35-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN423780.11947864142370002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAG4650.022.623711
GTATCGA41100.021.6295034
CCCGTAT48800.019.0806161
AAAGCCG62550.018.62780426
CGTATCG51500.018.1701093
CCGTATC54000.017.6184122
TGCATTG96200.017.38030245
CCGGTTA76000.016.7387630
GCCGGTT78900.016.15173329
AGCCGGT81100.015.85083228
GCGACAG93700.015.83248112
ATTCGGT14550.015.6911981
CGATTTC96800.014.95819919
CGACAGC97850.014.95611813
TCGAAGG70750.014.57675657
CGCTAGG11700.014.5394061
CGCGTAT1851.0274198E-714.51872351
AAGCCGG84950.013.89936227
GACAGCG108950.013.80037414
GCAAAAA89450.013.77334422
Produced by FastQC (version 0.11.5)