FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004973769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28516013
Sequences flagged as poor quality0
Sequence length35-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4176711.4646893308682387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAG4750.026.4598481
GTATCGA37650.020.5820584
CGACCGT13700.018.348071
CCCGTAT44550.018.0858821
CGTATCG48650.017.3014893
CCGTATC49750.017.0979792
CGCTAGG8700.016.2522771
CGCCAGT28100.016.213721
TCGAAGG57350.016.1527067
GGTATGT78550.015.44354645
CGCGTAA6600.015.3024691
CCGGTAA28750.014.9103721
CGTCCGT5050.014.2217011
GACCGTT13850.013.8268482
CGCGTAT1301.9701409E-413.81146051
CGCTCGT11900.013.7679361
GCGACAG66800.013.7336512
CGCGATT1504.3795244E-513.4661731
GTAGCGT25450.013.12433218
GTTGTCG17800.013.1131271
Produced by FastQC (version 0.11.5)