FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004973775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28186584
Sequences flagged as poor quality0
Sequence length35-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA32650.020.2620454
CCCGTAT36100.019.0302621
CGTATCG39500.017.3685513
CCGTATC44350.015.9211942
CGCGTAT1301.131496E-515.5428161
GGTATGT69850.015.094233545
TCGCATA12200.014.7892187
CGCGTAG6350.014.1421961
TCGAAGG56700.013.7107877
GTAGCGT24600.013.4005318
ATCGCAT13850.013.3489886
TCGATTG33050.013.00832325
TACTCGA34050.012.75635922
ATCGACC28350.012.75161743
GACCGTT13700.012.6818912
TAGCGTG26050.012.65464919
CGACCGT17200.012.26958751
ACTCGAT36050.012.17231823
TATTGCG29950.011.64691742
TCCCCGT87750.011.22668545
Produced by FastQC (version 0.11.5)