FastQCFastQC Report
Mon 13 May 2024
EGAF00004973778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63380069
Total Bases7.9 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT2699860.42597934060311615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1918000.30261879329919944No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT1404440.2215901658295765TruSeq Adapter, Index 10 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1056960.16676535962748792No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT1055160.16648135867444386RNA PCR Primer, Index 10 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT841940.13283986800329928No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)