FastQCFastQC Report
Mon 13 May 2024
EGAF00004973780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48265524
Total Bases6 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT1027100.2128019992075503No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT608870.12615008592882984TruSeq Adapter, Index 10 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570020.11810086222206974No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)