FastQCFastQC Report
Sat 25 May 2024
EGAF00004973812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78639211
Total Bases9.9 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT2399100.3050768146694656No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1308080.16633941050095225No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT1226190.1559260303361894TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT822250.10455979778332211RNA PCR Primer, Index 1 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)