FastQCFastQC Report
Sat 25 May 2024
EGAF00004973826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66917000
Total Bases8.4 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT1456900.2177174708967826No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT966720.1444655319276118RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT724060.10820269886575908RNA PCR Primer, Index 27 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)