FastQCFastQC Report
Sat 25 May 2024
EGAF00004973834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68002914
Total Bases8.5 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT4644910.6830457294815337No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT3003650.4416943073939449TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCT2286040.3361679471559116RNA PCR Primer, Index 1 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT791740.1164273636862091No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)