FastQCFastQC Report
Mon 13 May 2024
EGAF00004973836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61261834
Total Bases7.7 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT2186990.35699061833506324No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT1330410.21716783731939854RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT1021690.1667743084544286RNA PCR Primer, Index 27 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT942080.15377926818188303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)