FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004973840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57124488
Sequences flagged as poor quality0
Sequence length126
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGAACGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGG2785630.48764200739969865No Hit
GTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGT2571990.4502429851099935No Hit
AACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGG2423570.42426113298380896No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA2234460.39115624108525926No Hit
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG2098210.36730482380866153No Hit
CCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGT2081620.3644006402298083No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG1996290.34946308840439844No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGG1971530.3451286950703173No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT1863400.32619986020706215No Hit
CTGGAGGACAGGGCACTGGCCACTCCAGTCACCATCTTCTGCCAGGAAGC1750510.30643775748152No Hit
GAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGA1746200.3056832649423484No Hit
GAATAAAGCCTATCCTTGAAAGCTCTGCATCATGGGCAGTGAGCTCAGTG1706030.29865125443224977No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT1678730.29387221816325076No Hit
CGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGC1663070.2911308369188359No Hit
CCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGC1574870.2756908735882237No Hit
GTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAA1573290.27541428467595197No Hit
GCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGC1560980.2732593419480626No Hit
ACCTTACCCCAGGCGGCCTTGACGTTGGTCTTGTCGGCAGGAGACAGCAC1552740.27181687825368345No Hit
GACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCC1545370.27052671351732727No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA1538470.2693188252295583No Hit
ATCATGGGCAACCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGACTTC1487470.2603909552764832No Hit
GGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTC1450080.253845601206964No Hit
GGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTG1445750.25308760754232057No Hit
GACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACG1433110.2508748962441466No Hit
GTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCT1421290.2488057310903163No Hit
ACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTA1410170.2468591053279987No Hit
GCTTGTCACAGTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCA1360040.23808353433294668No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1328810.23261652690874007No Hit
ATACTCGCCAGCGTGCGCGCCGACCTTACCCCAGGCGGCCTTGACGTTGG1313950.230015190683197No Hit
CGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCA1300320.2276291736741693No Hit
TCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAACC1297870.2272002858038745No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC1257100.22006324152962212No Hit
CTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTG1252680.2192894928003556No Hit
GTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAAATGTG1207650.21140670880061105No Hit
GAACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCT1207330.21135069079306232No Hit
CCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGC1174020.20551956631978918No Hit
CCCATGGACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCT1148710.20108889203523367No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC1122560.1965111704808628No Hit
CCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGCC1122470.1964954154162397No Hit
CTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGCCGACCTTACCCC1109740.19426694905344272No Hit
CCTCAGGGGTGAATTCTTTGCCGAAATGGATTGCCAAAACGGTCACCAGC1093460.19141703291940226No Hit
GGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCA1082370.1894756588452924No Hit
GAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTAC1074630.18812072328770807No Hit
CTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTA1070860.18746076113627488No Hit
GGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCAC1056430.1849346991083754No Hit
GCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGC1050560.1839071187824038No Hit
CCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGG1032310.1807123417893916No Hit
CTGGAGGACAGGGCACTGGCCACTGCAGTCACCATCTTCTGCCAGGAAGC1029270.18018017071767892No Hit
GAATAAAGCCTATCCTTGAAAGCTCTGAATCATGGGCAAGAGGCTCAGTG1021330.17879022390537663No Hit
TCACAGTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAG1021220.17877096771528175No Hit
CCCTGGGAAGGCTCCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGAC1009050.176640532865695No Hit
CCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAG1007440.17635869226521558No Hit
TCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGC1000070.17506852752885943No Hit
CGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTG997730.1746588958486595No Hit
ATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAAC982950.17207156412500363No Hit
TCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTATGGC980610.1716619324448037No Hit
AAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTC970340.1698641045150374No Hit
GACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCC968880.16960852235559642No Hit
GGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATG959770.16801375970319418No Hit
TCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATA957750.167660146030543No Hit
AGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTG945420.16550170217718185No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC940370.16461766799555386No Hit
TCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAAT938910.1643620858361129No Hit
GGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCA925130.1619498103860467No Hit
GCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACC911180.15950777536947028No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG910880.15945525848739336No Hit
GCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACC903610.15818259937839618No Hit
GCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCA886770.1552346517311455No Hit
TTTCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTG885970.15509460671227374No Hit
CCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG882510.15448891200565334No Hit
CTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGT878530.15379218803676628No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT878460.153779934097615No Hit
TGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATG867830.15191908590935643No Hit
GGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCG867190.151807049894259No Hit
ATCATGGGCAGTGAGCTCAGTGGTATCTGGAGGACAGGGCACTGGCCACT859430.15044861321120287No Hit
GAATAGATTTATTATTTGATTGCTTGCAGAATAAAGCCTATCCTTGAAAG857210.1500599882838337No Hit
GTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGT855720.14979915443618505No Hit
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT844380.14781401629367777No Hit
CTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAG844090.14776324997433676No Hit
GACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGG837150.1465483594356242No Hit
GTGGTATCTGGAGGACAGGGCACTGGCCACTCCAGTCACCATCTTCTGCC828300.14499911141435526No Hit
GCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGAC811740.1421001795237097No Hit
GCTGAGTGAACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCA800270.14009228406563573No Hit
GGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAG792550.1387408496335232No Hit
CCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGACTTCCTTGGGAGATG792000.13864456868304886No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC788270.13799160878255923No Hit
GTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAG783750.13720035442593376No Hit
GTCAAGGCACATGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAA775890.13582441211551866No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC774640.13560559177353151No Hit
CCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAACCCTGGGAA755480.1322515135715527No Hit
GCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAA753270.13186463920691946No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA746190.1306252407899043No Hit
CCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGT741550.12981297968044808No Hit
GCTTTATGGCATCTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTG739340.1294261053158148No Hit
CCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGCCCAGCTGAGTGAA734730.12861909589456627No Hit
CTCCTAGTCCAGACGCCATGGGTCATTTCACAGAGGAGGACAAGGCTACT734160.12851931381862014No Hit
ATCCTTGAAAGCTCTGCATCATGGGCAGTGAGCTCAGTGGTATCTGGAGG733590.128419531742674No Hit
GCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGG730330.12784884829077156No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC723440.12664271056573848No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT723430.12664096000300257No Hit
GTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCA718970.12586020902279246No Hit
CCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAAT707880.1239188349486826No Hit
TTCTTGCCATGTGCCTTGACTTTGGGGTTGCCCATGATGGCAGAGGCAGA704720.12336565712413913No Hit
GAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCT700060.12254989488921109No Hit
ATGTGGAAGATGCTGGAGGAGAAACCCTGGGAAGGCTCCTGGTTGTCTAC699990.12253764095005981No Hit
GCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACC690970.12095863336228063No Hit
GTATCAACGCAGAGTACGGGGACTCTTCTGGTCCCCACAGACTCAGAGAG690610.12089561310378834No Hit
GTCCAGACGCCATGGGTCATTTCACAGAGGAGGACAAGGCTACTATCACA687050.12027241276980898No Hit
GTCCATGGGTAGACAACCAGGAGCCTTCCCAGGGTTTCTCCTCCAGCATC686020.12009210480801158No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT684780.11987503502876036No Hit
ATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAG677460.11859362310608368No Hit
GGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGC672150.11766407429332232No Hit
CCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACC667320.11681855249188404No Hit
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCT659610.1154688686225074No Hit
GTATCAACGCAGAGTACGGGACTCTTCTGGTCCCCACAGACTCAGAGAGA655700.11478439859277163No Hit
ACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCTCTGCCAT654670.11460409063097424No Hit
GGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTATGGCATCTCCCAAG654630.11459708838003065No Hit
CAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGG644720.11286228070875665No Hit
TCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGCA637860.11166139467193123No Hit
ACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCT633590.11091390438370317No Hit
GGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTA631600.11056554239925967No Hit
GCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTT627780.10989682743414697No Hit
CTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCC626810.10972702284876495No Hit
GAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGC623000.10906005844638818No Hit
GTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCAC618930.10834757941287808No Hit
GCACCATACTCGCCAGCGTGCGCGCCGACCTTACCCCAGGCGGCCTTGAC615740.1077891499001269No Hit
ACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGT614760.107617594752009No Hit
CTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGA614090.10750030704870386No Hit
GCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGA608700.10655675373405535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT608290.10648498066188355No Hit
GCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACCTCTGGGTCCATGGGTA605700.10603158491328621No Hit
GAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACG604510.10582326794771447No Hit
GCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTG603110.10557818916468889No Hit
CCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAG602100.10540138232836328No Hit
ACCAGGAGCCTTCCCAGGGTTTCTCCTCCAGCATCTTCCACATTCACCTT600210.10507052597127872No Hit
AGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGCAGTTCACT599320.10491472588778389No Hit
GTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGAC598460.10476417749249672No Hit
GTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACC590820.10342674756227137No Hit
ACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGA586510.10267225502309973No Hit
GTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTG586150.10260923476460744No Hit
GTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCA580500.10162016681882557No Hit
GCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAA579280.10140659816504614No Hit
CTCCAGTCACCATCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGAATTCT577500.10109499799805645No Hit
CCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTGCC576640.10094444960276931No Hit
GGCCACTCCAGTCACCATCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGA574020.10048580216596428No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)