FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004973841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57124488
Sequences flagged as poor quality0
Sequence length126
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG3510990.6146208260107294No Hit
CTTCTGGAACGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGG2985560.522641008178489No Hit
GTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGT2553140.4469431743528275No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGG2501570.43791552232380615No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA2157230.37763664507592615No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA2126060.3721801410281349No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG1974900.3457186347123146No Hit
GTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAA1930130.3378813653437034No Hit
GACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACG1893390.3314497978520175No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT1718980.3009182331752365No Hit
GTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGT1700450.29767444042561925No Hit
GGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCAC1664530.2913864190782769No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT1543470.27019410659750687No Hit
GACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCC1526510.2672251521974254No Hit
GCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGC1497660.26217477870436234No Hit
CGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCA1493340.26141853560245476No Hit
GTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCT1427700.24992784180402633No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC1406220.2461676330473194No Hit
CCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGT1401150.24528009774021958No Hit
CGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTG1391700.24362581595479682No Hit
GCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCC1381520.24184374308965362No Hit
CTGGAGGACAGGGCACTGGCCACTCCAGTCACCATCTTCTGCCAGGAAGC1371600.24010718485564367No Hit
TTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAG1371280.240051166848095No Hit
GAATAAAGCCTATCCTTGAAAGCTCTGCATCATGGGCAGTGAGCTCAGTG1366750.23925816192873361No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1348250.23601962086732403No Hit
GAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGA1333220.23338852507527072No Hit
GCTTGTCACAGTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCA1329760.2327828303686503No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC1300500.22766068380341542No Hit
CTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTG1299120.22741910614586164No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT1275020.22320024995234966No Hit
GTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAAATGTG1272400.22274160251554465No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG1265620.22155472098060644No Hit
ATCATGGGCAACCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGACTTC1260760.22070394749096042No Hit
GTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCA1242610.2175266761253072No Hit
TCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAACC1234860.21616999000498702No Hit
CCCATGGACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCT1220540.21366318416718239No Hit
GGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCA1206340.21117738508220854No Hit
CCTCAGGGGTGAATTCTTTGCCGAAATGGATTGCCAAAACGGTCACCAGC1205410.21101458274777007No Hit
GCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACC1202240.21045965436049074No Hit
GTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACC1179540.20648587695000437No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC1178610.2063230746155659No Hit
ACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTA1169470.20472306027495601No Hit
TCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGC1164070.20377775639757156No Hit
CCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGCC1160240.20310729086972298No Hit
CTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGCCGACCTTACCCC1160210.2031020391815153No Hit
GAACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCT1133490.1984245355511983No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA1073350.18789665125751323No Hit
ACCTTACCCCAGGCGGCCTTGACGTTGGTCTTGTCGGCAGGAGACAGCAC1071400.18755529152401332No Hit
TCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATA1070640.18742224875608512No Hit
CCCTGGGAAGGCTCCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGAC1055510.18477364733667284No Hit
GCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGC1032450.18073684966769418No Hit
CCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGC1009850.17678057788456678No Hit
CGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGC1003770.17571623574114134No Hit
CTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTA999120.1749022240689492No Hit
GTCCTCTGCCTCTGCCATCATGGGCAACCCCAAAGTCAAGGCACATGGCA990270.17335297604768027No Hit
TCACAGTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAG987610.17288732635993165No Hit
AACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGG983890.17223611702217795No Hit
GCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACC979040.17138709409526787No Hit
GTATCAACGCAGAGTACGGGGACTCTTCTGGTCCCCACAGACTCAGAGAG975890.17083566683346027No Hit
GGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATG974980.17067636562449365No Hit
GGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTC973610.17043653852967575No Hit
TTTCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTG972260.17020021256032963No Hit
GGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGAC967270.169326681755117No Hit
TCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTATGGC943210.16511482781254863No Hit
CCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG928460.16253274777710042No Hit
GTATCAACGCAGAGTACGGGACTCTTCTGGTCCCCACAGACTCAGAGAGA919160.16090472443271614No Hit
TCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAAT913850.15997517561995478No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC904030.15825612301330386No Hit
CTCCTAGTCCAGACGCCATGGGTCATTTCACAGAGGAGGACAAGGCTACT900480.1576346732420604No Hit
GCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCA898270.15724779887742713No Hit
GACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCC894090.15651606365382215No Hit
GTCCAGACGCCATGGGTCATTTCACAGAGGAGGACAAGGCTACTATCACA893780.15646179620900935No Hit
GCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAA891140.15599964764673252No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC868410.15202061854803847No Hit
CCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGACTTCCTTGGGAGATG862490.15098428540838738No Hit
ATACTCGCCAGCGTGCGCGCCGACCTTACCCCAGGCGGCCTTGACGTTGG861150.15074971000177717No Hit
AGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTG853760.14945604413994923No Hit
GAATAAAGCCTATCCTTGAAAGCTCTGAATCATGGGCAAGAGGCTCAGTG847920.14843371550218531No Hit
GTCCATGGGTAGACAACCAGGAGCCTTCCCAGGGTTTCTCCTCCAGCATC844110.14776675109980855No Hit
GGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAG837510.14661137969411647No Hit
TGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATG836000.14634704472099602No Hit
GCTTTATGGCATCTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTG830680.14541574534549878No Hit
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT829730.14524944188558855No Hit
GCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACC825020.14442492683698102No Hit
GACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGG824810.14438816501952717No Hit
GGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCG820090.14356189940818376No Hit
ATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAAC819020.14337458919544277No Hit
GAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTAC818680.14331507006242228No Hit
CTGGAGGACAGGGCACTGGCCACTGCAGTCACCATCTTCTGCCAGGAAGC817350.14308224521854795No Hit
GTCAAGGCACATGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAA811700.1420931772727661No Hit
CTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGT811500.14205816601804816No Hit
CCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGC802220.14043364379913567No Hit
GAATAGATTTATTATTTGATTGCTTGCAGAATAAAGCCTATCCTTGAAAG801740.1403496167878126No Hit
GTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAG797350.13958111974675377No Hit
GCTGAGTGAACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCA797150.13954610849203586No Hit
GTGGTATCTGGAGGACAGGGCACTGGCCACTCCAGTCACCATCTTCTGCC797050.13952860286467686No Hit
GTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGG784190.13727737918631322No Hit
GTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCG779460.1364493630122339No Hit
CCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACC778280.13624279660939806No Hit
GCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGAC776400.1359136908150494No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC761830.13336312090884736No Hit
CCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGG761580.13331935684044993No Hit
GGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCA758930.13285545771543722No Hit
GGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGC756540.1324370732215578No Hit
GGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACT750560.13139023670549135No Hit
CTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGA738070.12920378384835587No Hit
GTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCA733020.12831974966672788No Hit
CCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGCCCAGCTGAGTGAA726100.1271083602534871No Hit
GTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCAC721760.12634861602610775No Hit
GCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTT721710.12633986321242827No Hit
CTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAG718570.12579018651335658No Hit
TTCTTGCCATGTGCCTTGACTTTGGGGTTGCCCATGATGGCAGAGGCAGA712960.12480812081851832No Hit
ACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCT706650.12370351573216727No Hit
ATCATGGGCAGTGAGCTCAGTGGTATCTGGAGGACAGGGCACTGGCCACT700030.12254464320100339No Hit
TTCCCAGGGTTTCTCCTCCAGCATCTTCCACATTCACCTTGCCCCACAGG697010.12201597325476247No Hit
TCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGCA690870.12094112773492166No Hit
CCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAACCCTGGGAA687280.12031267571273462No Hit
GGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTG681030.11921857400279894No Hit
GGCCACTCCAGTCACCATCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGA678000.11868815349382211No Hit
GGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTA670780.11742424719850444No Hit
CCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAAT665300.11646493881923284No Hit
ACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCTCTGCCAT658080.11520103252391513No Hit
CCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGT654700.11460934231918192No Hit
GCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGA653610.11441853098096914No Hit
ATCCTTGAAAGCTCTGCATCATGGGCAGTGAGCTCAGTGGTATCTGGAGG653120.11433275340691018No Hit
GGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAG652360.114199710638982No Hit
CTCCAGTCACCATCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGAATTCT652150.11416294882152817No Hit
GGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTATGGCATCTCCCAAG649910.11377082276868722No Hit
GAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCT645170.112941056031872No Hit
CCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAG634260.1110311920870083No Hit
GTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGAC629570.11021017816387256No Hit
CTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACC628830.11008063652141618No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT626860.10973577566244445No Hit
CTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAA625410.10948194406573937No Hit
GAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACG613160.10733750471426545No Hit
GCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCTTGTCACAGTGCA612660.1072499765774706No Hit
GCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGA611920.1071204349350142No Hit
TCTCTTAGCAGAATAGATTTATTATTTGATTGCTTGCAGAATAAAGCCTA610760.10691736965765014No Hit
GGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCG608870.1065865133005656No Hit
GCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTG600170.10506352372033514No Hit
GCTCCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGACAGCTTTGGCA598160.10471166061041984No Hit
GCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAA597220.1045471077132455No Hit
GAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGC591590.10356154089293546No Hit
GGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGAT590480.10336722842925086No Hit
CCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAG590140.10330770929623037No Hit
GTGCCTTGACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTG585400.10247794255941514No Hit
GTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTG584910.1023921649853562No Hit
GTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACG580290.10158340500137172No Hit
CCTCTGGGTCCATGGGTAGACAACCAGGAGCCTTCCCAGGGTTTCTCCTC577990.10118077557211541No Hit
GGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCC576010.1008341641504078No Hit
GTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCC574760.10061534380842066No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)