FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004973845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60406826
Sequences flagged as poor quality0
Sequence length126
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGAACGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGG2853070.4723092055854747No Hit
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG2620230.4337638928421764No Hit
GTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGT2339890.38735523035095404No Hit
GAATAAAGCCTATCCTTGAAAGCTCTGCATCATGGGCAGTGAGCTCAGTG2259150.3739891912215351No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGG2098960.3474706649874304No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA1953320.3233608069392688No Hit
GACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACG1930240.31954004668280367No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG1834680.30372064243203245No Hit
TCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAACC1746290.28908819013268466No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT1650560.2732406433670261No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA1581630.26182968130124895No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC1569250.2597802440406321No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC1541660.2552128794186273No Hit
GTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGT1532070.2536253104905727No Hit
GAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGA1530070.2532942220801338No Hit
GTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAAATGTG1484900.24581659033037095No Hit
CTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTG1461910.24201072905237564No Hit
GTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCT1433540.23731423995029965No Hit
CTGGAGGACAGGGCACTGGCCACTCCAGTCACCATCTTCTGCCAGGAAGC1394400.2308348397580101No Hit
ATCATGGGCAACCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGACTTC1380030.22845596952900654No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC1379240.22832518960688317No Hit
TTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAG1369220.22666643667058423No Hit
GTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAA1363090.22565165069258894No Hit
CGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCA1358150.22483386231880484No Hit
CCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGCC1356720.22459713410534102No Hit
CCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGT1349510.22340356038570872No Hit
CCTCAGGGGTGAATTCTTTGCCGAAATGGATTGCCAAAACGGTCACCAGC1343870.222469891068271No Hit
CCCATGGACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCT1341230.22203285436649162No Hit
GCTTGTCACAGTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCA1319540.2184422005552816No Hit
GAATAAAGCCTATCCTTGAAAGCTCTGAATCATGGGCAAGAGGCTCAGTG1310290.21691091665700163No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1300950.21536473378025192No Hit
GTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCA1295660.214489004934641No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT1289510.21347090807254135No Hit
GGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCAC1287130.21307691286411903No Hit
GCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGC1265740.20953592231447485No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT1254640.2076983816365389No Hit
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT1253420.20749641770617117No Hit
TCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGC1239460.20518542060130757No Hit
TCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAAT1237960.20493710429347836No Hit
TTTCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTG1233510.20420043258025178No Hit
AACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGG1232640.20405640912171086No Hit
GACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCC1202570.1990784948707618No Hit
TCACAGTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAG1199260.1985305435514854No Hit
GTCCTCTGCCTCTGCCATCATGGGCAACCCCAAAGTCAAGGCACATGGCA1187550.19659202090836556No Hit
CGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTG1159630.19197002669863833No Hit
GTATCAACGCAGAGTACGGGGACTCTTCTGGTCCCCACAGACTCAGAGAG1151470.19061918598404756No Hit
CTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGCCGACCTTACCCC1140830.18885779564051255No Hit
GTATCAACGCAGAGTACGGGACTCTTCTGGTCCCCACAGACTCAGAGAGA1138770.18851677457776048No Hit
TCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTATGGC1133700.18767746545729783No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG1126030.18640774140326458No Hit
GCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCC1090430.18051436769745194No Hit
ATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAAC1082660.17922808922289676No Hit
CTCCTAGTCCAGACGCCATGGGTCATTTCACAGAGGAGGACAAGGCTACT1075430.17803120461916008No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA1074050.17780275361595724No Hit
GTCCAGACGCCATGGGTCATTTCACAGAGGAGGACAAGGCTACTATCACA1063230.17601156531548273No Hit
GCTTTATGGCATCTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTG1061350.17570034220967012No Hit
GCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACC1054040.1744902140695159No Hit
GTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACC1051650.17409456341904142No Hit
CCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGACTTCCTTGGGAGATG1047920.17347708353357283No Hit
GCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAA1044220.17286456997426086No Hit
GAACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCT1042590.17259473291975314No Hit
GGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAG1038830.171972286708128No Hit
GAATAGATTTATTATTTGATTGCTTGCAGAATAAAGCCTATCCTTGAAAG1033880.17115284289229168No Hit
GTCAAGGCACATGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAA1023950.16950898893446248No Hit
ACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTA1008160.16689504593404725No Hit
GTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGG987890.1635394648942489No Hit
GGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATG987320.16344510469727377No Hit
TCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATA982490.16264552618606382No Hit
CCCTGGGAAGGCTCCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGAC973310.1611258303821492No Hit
GGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCA959940.15891250435836507No Hit
TCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGCA932040.15429382103274222No Hit
CCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGC911610.15091175292010875No Hit
GCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGC902160.14934736018078487No Hit
CCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGCCCAGCTGAGTGAA889540.14725819231091533No Hit
TCTCTTAGCAGAATAGATTTATTATTTGATTGCTTGCAGAATAAAGCCTA888310.1470545729384954No Hit
GCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACC878340.14540409721245742No Hit
CTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACC876890.1451640581148892No Hit
GCTGAGTGAACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCA875720.14497037139478244No Hit
GGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTC873230.14455816632378599No Hit
GCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCA872950.14451181394632454No Hit
GCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTT870400.14408967622301494No Hit
GACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGG869790.14398869425783106No Hit
CCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG868620.14379500753772428No Hit
GTGGTATCTGGAGGACAGGGCACTGGCCACTCCAGTCACCATCTTCTGCC868510.14377679767515014No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC862120.1427189702037978No Hit
GCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAA856700.14182172061150838No Hit
GGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGC853530.1412969454809627No Hit
GTCCATGGGTAGACAACCAGGAGCCTTCCCAGGGTTTCTCCTCCAGCATC852610.1411446448121608No Hit
ATCATGGGCAGTGAGCTCAGTGGTATCTGGAGGACAGGGCACTGGCCACT842910.1395388660215321No Hit
ACCTTACCCCAGGCGGCCTTGACGTTGGTCTTGTCGGCAGGAGACAGCAC840490.139138249044901No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC837310.1386118184723031No Hit
ATACTCGCCAGCGTGCGCGCCGACCTTACCCCAGGCGGCCTTGACGTTGG835720.13834860318600417No Hit
GCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACC832960.13789170117959848No Hit
TGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATG822380.1361402434883766No Hit
CTGGAGGACAGGGCACTGGCCACTGCAGTCACCATCTTCTGCCAGGAAGC817450.1353241105566447No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC816770.1352115404970955No Hit
GACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCC804610.13319852296162688No Hit
TTCTTGCCATGTGCCTTGACTTTGGGGTTGCCCATGATGGCAGAGGCAGA801430.13267209238902902No Hit
CTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTA797090.13195363053837655No Hit
GCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGAC793240.13131628534828166No Hit
AGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTG787970.13044386738677513No Hit
GGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACT787620.13038592691494832No Hit
CCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAG784120.12980652219668024No Hit
GGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGAC779660.12906819504140146No Hit
GATTTATTATTTGTATTGCTTGCAGAATAAAGCCTATCCTTGAAAGCTCT775420.12836628761127095No Hit
GTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCAC770310.12752035672259954No Hit
GGCTTGTGATAGTAGCCTTGTCCTCCTCTGTGAAATGACCCATGGCGTCT769830.12744089550409418No Hit
GGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCG769460.127379644148163No Hit
GGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTG764800.12660820815184035No Hit
CCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAAT764100.1264923272081867No Hit
ACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCT759190.12567950516055917No Hit
GTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCG758460.12555865789074896No Hit
GTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAG758050.125490784766609No Hit
GATTTATTATTTGATTGCTTGCAGAATAAAGCCTATCCTTGAAAGCTCTG757300.12536662661269438No Hit
CTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCC757230.12535503851832905No Hit
CTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAG756660.12526067832135396No Hit
GTATCAACGCAGAGTACGGGACACTCGCTTCTGGAACGTCTGAGGTTATC752340.1245455273548059No Hit
CTTGAAAGCTCTGCATCATGGGCAGTGAGCTCAGTGGTATCTGGAGGACA747150.12368635292971691No Hit
ATCCTTGAAAGCTCTGCATCATGGGCAGTGAGCTCAGTGGTATCTGGAGG746990.1236598658568818No Hit
TTCCCAGGGTTTCTCCTCCAGCATCTTCCACATTCACCTTGCCCCACAGG746670.12360689171121157No Hit
CCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGC745540.1234198267593136No Hit
CTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGA744830.12330229037360778No Hit
CCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACC741430.12273944007586163No Hit
CCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAACCCTGGGAA740080.12251595539881535No Hit
GTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCA737990.1221699680099067No Hit
GTGCCTTGACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTG735710.12179252722200633No Hit
CTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAA721010.11935902740528033No Hit
CTCCAGTCACCATCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGAATTCT720970.11935240563707154No Hit
CCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGT711820.11783767615931352No Hit
GGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTA704840.11668217760688171No Hit
CGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGC702250.11625341811536331No Hit
GGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTATGGCATCTCCCAAG701050.11605476506909997No Hit
ACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCTCTGCCAT699900.11586438923309761No Hit
GAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGC693520.11480821720379747No Hit
TATCAACGCAGAGTACGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAA688480.11397387440949142No Hit
GAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTAC685770.11352524961334667No Hit
CTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGT685640.11350372886666815No Hit
GACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCC680460.11264620988363136No Hit
CCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTGCC679010.11240617078606314No Hit
ATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAG675430.1118135225313775No Hit
CCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAG673750.11153540826660882No Hit
GTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTT665000.11008689647093856No Hit
GTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTG663730.10987665533030985No Hit
CACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGG662210.10962502813837628No Hit
TATCAACGCAGAGTACGGGGACTCTTCTGGTCCCCACAGACTCAGAGAGA662020.1095935747393846No Hit
CCTCTGGGTCCATGGGTAGACAACCAGGAGCCTTCCCAGGGTTTCTCCTC661590.10952239073114022No Hit
ACCAGGAGCCTTCCCAGGGTTTCTCCTCCAGCATCTTCCACATTCACCTT658650.10903569076779501No Hit
ACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCT657240.10880227343843557No Hit
GGCCACTCCAGTCACCATCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGA652140.10795799799181635No Hit
CCTTCTTGCCATGTGCCTTGACTTTGGGGTTGCCCATGATGGCAGAGGCA645220.10681243209169772No Hit
CTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCG644300.10666013142289581No Hit
GCCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGCCCAGCTGAGTGA642930.10643333586174517No Hit
CCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGG641370.10617508690160282No Hit
GAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGG627840.10393527380498356No Hit
GCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGAT625670.10357604287965735No Hit
TCCACATGCAGCTTGTCACAGTGCAGTTCACTCAGCTGGGCAAAGGTGCC621380.10286585823926589No Hit
GGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAG619810.10260595383707131No Hit
GAATAGATTTATTATTTGTATTGCTTGCAGAATAAAGCCTATCCTTGAAA617840.102279831752789No Hit
TCCAGGTGCTTTATGGCATCTCCCAAGGAAGTCAGCACCTTCTTGCCATG612060.10132298624662055No Hit
TCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAGGGTT610020.10098527606797285No Hit
GTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACG608560.10074358152835244No Hit
GCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGA606470.10039759413944377No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)