FastQCFastQC Report
Wed 22 May 2024
EGAF00004973846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63403945
Total Bases7.9 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT1680610.2650639483079483No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT1163590.18352012638961188TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCT943140.14875099648767912RNA PCR Primer, Index 1 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT878510.13855762445065525No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)