FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004973852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66043626
Sequences flagged as poor quality0
Sequence length126
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGAACGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGG3103890.46997570969225705No Hit
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG2424320.36707857318433723No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGG2235030.33841721531158814No Hit
GAATAAAGCCTATCCTTGAAAGCTCTGCATCATGGGCAGTGAGCTCAGTG2206020.33402466424239036No Hit
CCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGT2114930.32023226586620185No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1978510.29957622254114274No Hit
TCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAACC1675370.2536762593864849No Hit
CGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCA1665000.25210608515044286No Hit
CTGGAGGACAGGGCACTGGCCACTCCAGTCACCATCTTCTGCCAGGAAGC1657590.2509840995102237No Hit
GTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGT1596700.2417644361319592No Hit
AACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGG1584440.2399080874208815No Hit
GCTTGTCACAGTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCA1522380.2305112684152139No Hit
GAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGA1513690.2291954714903146No Hit
CCTCAGGGGTGAATTCTTTGCCGAAATGGATTGCCAAAACGGTCACCAGC1500680.22722556147961956No Hit
CTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTG1484460.22476960910656238No Hit
GTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAAATGTG1476270.2235295197147413No Hit
CCCATGGACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCT1466160.22199871339589985No Hit
CCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGCC1434200.2171594878815406No Hit
GACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACG1379640.2088982818720462No Hit
CCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGACTTCCTTGGGAGATG1321470.20009046747372713No Hit
TCACAGTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAG1278210.19354025171180034No Hit
CCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGC1270300.19234255853850302No Hit
GTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCT1264620.19148252096273455No Hit
TTTCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTG1262480.19115849272115978No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT1261680.19103736066823468No Hit
CTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGCCGACCTTACCCC1261460.19100404935368026No Hit
TCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAAT1247980.18896297426189168No Hit
CCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAG1233100.1867099180774841No Hit
GCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGC1227560.18587107861097754No Hit
ATCATGGGCAACCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGACTTC1214600.18390873935359028No Hit
TCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTATGGC1193150.18066088618453505No Hit
CGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTG1192790.18060637676071872No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA1191560.18042013622934633No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1183900.17926029682258815No Hit
GCTTTATGGCATCTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTG1169400.17706477836332002No Hit
GGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTG1148860.17395471290446712No Hit
GTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAA1124670.1702919824541433No Hit
CTCCTAGTCCAGACGCCATGGGTCATTTCACAGAGGAGGACAAGGCTACT1123990.17018902020915688No Hit
GGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATG1102610.16695176609473258No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1077960.1632193847139768No Hit
GGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAG1069780.16198080947281726No Hit
CCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGCCCAGCTGAGTGAA1068520.16179002648946017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1066410.1614705406998701No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC1065580.16134486619496027No Hit
GGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTC1054250.15962933349540803No Hit
GCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAA1045140.158249942242723No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG1036830.15699168304296315No Hit
GGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCA1023220.15493092399257424No Hit
ATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAAC1017700.15409511282739077No Hit
GTCAAGGCACATGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAA1009640.15287470739417003No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA994810.1506292219630703No Hit
CCCTGGGAAGGCTCCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGAC984010.14899393924858093No Hit
GAATAAAGCCTATCCTTGAAAGCTCTGAATCATGGGCAAGAGGCTCAGTG973200.14735714238343No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT972700.1472814348503518No Hit
GGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCG971480.14709670846964096No Hit
CCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGC967180.14644562368516834No Hit
GTCCTCTGCCTCTGCCATCATGGGCAACCCCAAAGTCAAGGCACATGGCA966640.1463638595494439No Hit
ATACTCGCCAGCGTGCGCGCCGACCTTACCCCAGGCGGCCTTGACGTTGG963830.14593838321354433No Hit
GACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCC938090.14204095941067804No Hit
ACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCT937680.1419788792335539No Hit
CCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAAT928380.14057071911829916No Hit
GAACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCT927390.1404208182028043No Hit
CTTGAAAGCTCTGCATCATGGGCAGTGAGCTCAGTGGTATCTGGAGGACA912170.13811628089590355No Hit
GGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGC909420.1376998894639734No Hit
TGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATG899980.1362705312394568No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT897030.13582385679429534No Hit
GCTGAGTGAACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCA888290.1345004891160882No Hit
ACCTTACCCCAGGCGGCCTTGACGTTGGTCTTGTCGGCAGGAGACAGCAC888020.134459607048226No Hit
GTGGTATCTGGAGGACAGGGCACTGGCCACTCCAGTCACCATCTTCTGCC885240.13403867316431112No Hit
GTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGT883310.13374644208662922No Hit
GGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCAC883300.13374492793596765No Hit
CCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAACCCTGGGAA881180.13342392799571604No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC873110.13220200841183372No Hit
TCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGCA869820.1317038528441791No Hit
GTGCCTTGACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTG867580.1313646830959887No Hit
GGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTATGGCATCTCCCAAG850290.12874671660214415No Hit
TCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATA847810.12837120723807624No Hit
CTGGAGGACAGGGCACTGGCCACTGCAGTCACCATCTTCTGCCAGGAAGC847760.1283636364847684No Hit
CGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGC843000.12764290076986384No Hit
GTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCA841860.1274702875944455No Hit
GCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACC839580.1271250612436089No Hit
GCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCA833630.1262241415999782No Hit
GCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGC829670.12562453793799874No Hit
GCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGAC820810.12428300045185285No Hit
CTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGA819440.12407556181121854No Hit
ATCATGGGCAGTGAGCTCAGTGGTATCTGGAGGACAGGGCACTGGCCACT816100.12356983549025607No Hit
GATTTATTATTTGTATTGCTTGCAGAATAAAGCCTATCCTTGAAAGCTCT811590.12288695354189064No Hit
GTCCAGACGCCATGGGTCATTTCACAGAGGAGGACAAGGCTACTATCACA809050.12250235927385332No Hit
AGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTG796530.12060664264557491No Hit
CCTTCTTGCCATGTGCCTTGACTTTGGGGTTGCCCATGATGGCAGAGGCA783810.11868064300406522No Hit
CCTCTGGGTCCATGGGTAGACAACCAGGAGCCTTCCCAGGGTTTCTCCTC783040.11856405340312477No Hit
GACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGG778270.11784180353755865No Hit
GGCTTGTGATAGTAGCCTTGTCCTCCTCTGTGAAATGACCCATGGCGTCT776560.11758288377443117No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG775240.11738301588710467No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC775150.11736938853115061No Hit
GGTGAATTCTTTGCCGAAATGGATTGCCAAAACGGTCACCAGCACATTTC773840.1171710347944857No Hit
GTCCATGGGTAGACAACCAGGAGCCTTCCCAGGGTTTCTCCTCCAGCATC768810.11640941701171889No Hit
TCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGC765940.11597485577184996No Hit
CTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCG764690.11578558693915444No Hit
GCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTT762850.11550698321742663No Hit
CCTTTGCCCAGCTGAGTGAACTGCACTGTGACAAGCTGCATGTGGATCCT760280.11511784649740461No Hit
GCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACC754360.11422146930575858No Hit
TTCTTGCCATGTGCCTTGACTTTGGGGTTGCCCATGATGGCAGAGGCAGA752790.11398374765189301No Hit
CCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGG752690.11396860614527736No Hit
CTCCAGTCACCATCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGAATTCT750550.11364457790370264No Hit
ACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCTCTGCCAT742050.11235754984137303No Hit
GGCCACTCCAGTCACCATCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGA741230.11223338948712477No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT735190.11131884248753998No Hit
GCACCATACTCGCCAGCGTGCGCGCCGACCTTACCCCAGGCGGCCTTGAC733280.11102963971118121No Hit
CCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGT727930.11021956910724434No Hit
TCTCTTAGCAGAATAGATTTATTATTTGATTGCTTGCAGAATAAAGCCTA717340.10861608355664785No Hit
GAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTAC701260.10618132929285258No Hit
GATTTATTATTTGATTGCTTGCAGAATAAAGCCTATCCTTGAAAGCTCTG698580.10577553691555337No Hit
GAATAGATTTATTATTTGATTGCTTGCAGAATAAAGCCTATCCTTGAAAG692820.10490338613449238No Hit
AGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGCAGTTCACT690230.10451122111314726No Hit
GGTGAATGTGGAAGATGCTGGAGGAGAAACCCTGGGAAGGCTCCTGGTTG687980.1041705372142953No Hit
GCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGAT686200.10390101839653684No Hit
ACCAGGAGCCTTCCCAGGGTTTCTCCTCCAGCATCTTCCACATTCACCTT679900.10294710347975139No Hit
GACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCC679360.10286533934402693No Hit
GCTCCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGACAGCTTTGGCA675570.1022914762432941No Hit
GGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCA672600.10184177349680952No Hit
GCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACCTCTGGGTCCATGGGTA667460.10106350005676551No Hit
CCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTGCC665160.10071524540460573No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)