FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004973853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66043626
Sequences flagged as poor quality0
Sequence length126
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGAACGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGG2756530.4173801723121623No Hit
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG2727150.4129315976684865No Hit
GAATAAAGCCTATCCTTGAAAGCTCTGCATCATGGGCAGTGAGCTCAGTG2357410.35694739110781104No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGG2165520.3278923540630552No Hit
GACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACG1961870.2970566758402999No Hit
TCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAACC1660930.2514898258311862No Hit
GAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGA1529860.2316438531100639No Hit
GTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAAATGTG1511590.22887749985138608No Hit
GCTTGTCACAGTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCA1488330.2253555854125877No Hit
CTGGAGGACAGGGCACTGGCCACTCCAGTCACCATCTTCTGCCAGGAAGC1445050.2188023413493378No Hit
CCTCAGGGGTGAATTCTTTGCCGAAATGGATTGCCAAAACGGTCACCAGC1437090.21759707742273265No Hit
GTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGT1403780.21255344156906225No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA1399170.21185541811408115No Hit
CGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCA1390440.21053356458653558No Hit
CCCATGGACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCT1382700.20936161197448486No Hit
CTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTG1379390.20886042810550712No Hit
TTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAG1377700.20860453664370274No Hit
GTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCA1357180.20549749948617296No Hit
CCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGT1335070.2021497123734545No Hit
CCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGACTTCCTTGGGAGATG1333310.20188322185701918No Hit
GTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAA1324530.20055379757616582No Hit
CCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGCC1320930.2000087033380027No Hit
TCACAGTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAG1296640.1963308313810632No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1282230.19414894027774912No Hit
GTCCTCTGCCTCTGCCATCATGGGCAACCCCAAAGTCAAGGCACATGGCA1281610.19405506293673216No Hit
GTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGT1280440.19387790730932913No Hit
GTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCT1261150.19095711068317175No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT1251580.1895080685000548No Hit
GGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCAC1247060.18882367240102776No Hit
TCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAAT1245510.18858897904848532No Hit
TTTCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTG1242450.18812564894604666No Hit
CGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTG1238860.18758206885854511No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT1225470.18555462112271062No Hit
GCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCC1212160.18353928659216862No Hit
ATCATGGGCAACCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGACTTC1194050.18079715974407584No Hit
CTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGCCGACCTTACCCC1187750.1798432448272904No Hit
GCTTTATGGCATCTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTG1182130.17899229215549128No Hit
GCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGC1149310.1740228496842375No Hit
GTCAAGGCACATGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAA1140520.17269191125272254No Hit
GACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCC1133370.17160929352970414No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC1130340.17115050587925018No Hit
GCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAA1129700.17105360023691007No Hit
CTCCTAGTCCAGACGCCATGGGTCATTTCACAGAGGAGGACAAGGCTACT1122520.16996644006190698No Hit
TCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTATGGC1110620.16816460077464554No Hit
CCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGCCCAGCTGAGTGAA1037670.15711887169853456No Hit
GAATAAAGCCTATCCTTGAAAGCTCTGAATCATGGGCAAGAGGCTCAGTG1034990.15671307932123535No Hit
GGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATG1028180.1556819427207101No Hit
GGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAG1025180.15522769752224083No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC1022640.1548431032542035No Hit
ATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAAC1007180.15250222633142524No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA1004140.1520419245303097No Hit
GAACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCT989170.14977524098994807No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG981930.14867899591097555No Hit
GAATAGATTTATTATTTGATTGCTTGCAGAATAAAGCCTATCCTTGAAAG968190.1465985529019863No Hit
GCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACC953900.14443483160661105No Hit
CCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGC950570.1439306194363102No Hit
GTCCAGACGCCATGGGTCATTTCACAGAGGAGGACAAGGCTACTATCACA950010.14384582699926257No Hit
GATTTATTATTTGTATTGCTTGCAGAATAAAGCCTATCCTTGAAAGCTCT948230.14357630818150416No Hit
ACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCT945470.1431584025989124No Hit
CCCTGGGAAGGCTCCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGAC942510.14271021400308942No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC941790.14260119515545677No Hit
GCTGAGTGAACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCA939980.14232713388571366No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG939400.14223931314734295No Hit
GCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTT932540.1412006057935099No Hit
TCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGCA923720.13986512491001024No Hit
GTCCATGGGTAGACAACCAGGAGCCTTCCCAGGGTTTCTCCTCCAGCATC912900.13822681389419775No Hit
GTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGG909370.1376923187106656No Hit
AACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGG907960.13747882346738502No Hit
GGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCG886360.1342082580384063No Hit
GTGGTATCTGGAGGACAGGGCACTGGCCACTCCAGTCACCATCTTCTGCC873280.13222774897308032No Hit
GACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGG869700.13168568303624031No Hit
GCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCA858040.12992018336485642No Hit
GTGCCTTGACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTG852900.12914190992481242No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT852180.1290328910771798No Hit
GGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCA847260.1282879289516902No Hit
GCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGAC845480.12801841013393178No Hit
CCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAAT841300.12738549515739794No Hit
GTATCAACGCAGAGTACGGGACTCTTCTGGTCCCCACAGACTCAGAGAGA839140.12705843861450006No Hit
ATCATGGGCAGTGAGCTCAGTGGTATCTGGAGGACAGGGCACTGGCCACT837100.12674955187954096No Hit
TCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATA834920.12641946703531995No Hit
ATACTCGCCAGCGTGCGCGCCGACCTTACCCCAGGCGGCCTTGACGTTGG833240.12616508972417717No Hit
TCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGC832930.1261181510536687No Hit
GACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCC832750.12609089634176052No Hit
GTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACC826090.12508247200115874No Hit
ACCTTACCCCAGGCGGCCTTGACGTTGGTCTTGTCGGCAGGAGACAGCAC824890.12490077392177104No Hit
ACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCTCTGCCAT820820.1242845146025144No Hit
CTTGAAAGCTCTGCATCATGGGCAGTGAGCTCAGTGGTATCTGGAGGACA817640.12380301469213698No Hit
GCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGC813670.123201896879496No Hit
GCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACC802160.12145910946803556No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC799250.12101849162552039No Hit
TGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATG795730.12048551059264977No Hit
GATTTATTATTTGATTGCTTGCAGAATAAAGCCTATCCTTGAAAGCTCTG794990.12037346344369401No Hit
GGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACT767170.11616109630322236No Hit
GGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTATGGCATCTCCCAAG761190.11525563420760696No Hit
GGCTTGTGATAGTAGCCTTGTCCTCCTCTGTGAAATGACCCATGGCGTCT757680.1147241673253979No Hit
TTCTTGCCATGTGCCTTGACTTTGGGGTTGCCCATGATGGCAGAGGCAGA753240.1140518844316634No Hit
AGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTG749610.11350224774151559No Hit
GTATCAACGCAGAGTACGGGGACTCTTCTGGTCCCCACAGACTCAGAGAG748980.11340685624983704No Hit
CCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAACCCTGGGAA748620.11335234682602073No Hit
CTCCAGTCACCATCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGAATTCT744680.11275577146536442No Hit
CTGGAGGACAGGGCACTGGCCACTGCAGTCACCATCTTCTGCCAGGAAGC738150.111767031083363No Hit
CCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGC725640.10987282860574615No Hit
GGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGC724890.10975926730612882No Hit
CCTCTGGGTCCATGGGTAGACAACCAGGAGCCTTCCCAGGGTTTCTCCTC722460.10939132869536873No Hit
GGTGAATTCTTTGCCGAAATGGATTGCCAAAACGGTCACCAGCACATTTC722090.10933530512089085No Hit
CCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAG721550.10925354098516639No Hit
CTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGA718510.10879323918405086No Hit
CCTTCTTGCCATGTGCCTTGACTTTGGGGTTGCCCATGATGGCAGAGGCA712690.10791200349902048No Hit
GAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTAC706960.10704439516994418No Hit
GCTCCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGACAGCTTTGGCA699640.10593603688567918No Hit
GCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACCTCTGGGTCCATGGGTA699430.10590423972178632No Hit
CTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCG698280.10573011239570643No Hit
CCTTTGCCCAGCTGAGTGAACTGCACTGTGACAAGCTGCATGTGGATCCT696360.10543939546868611No Hit
TCTCTTAGCAGAATAGATTTATTATTTGATTGCTTGCAGAATAAAGCCTA695590.10532280586774566No Hit
GAATAGATTTATTATTTGTATTGCTTGCAGAATAAAGCCTATCCTTGAAA692750.10489278707986142No Hit
GGCCACTCCAGTCACCATCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGA685250.10375717408368827No Hit
GCACCATACTCGCCAGCGTGCGCGCCGACCTTACCCCAGGCGGCCTTGAC678950.10280325916690279No Hit
TTCCCAGGGTTTCTCCTCCAGCATCTTCCACATTCACCTTGCCCCACAGG674540.10213551872515299No Hit
ACCAGGAGCCTTCCCAGGGTTTCTCCTCCAGCATCTTCCACATTCACCTT672490.10182511783953233No Hit
GGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGAC668840.10127245284806137No Hit
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT666360.10089694348399345No Hit
GCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGAT664450.10060774070763467No Hit
ATCCTTGAAAGCTCTGCATCATGGGCAGTGAGCTCAGTGGTATCTGGAGG662480.10030945302730652No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)