FastQCFastQC Report
Wed 22 May 2024
EGAF00004973858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71116787
Total Bases8.9 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT1481140.20826868907899337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1044610.1468865571781245No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT767200.10787888941045665RNA PCR Primer, Index 17 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)