FastQCFastQC Report
Sat 25 May 2024
EGAF00004973866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69771666
Total Bases8.7 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT2295500.3290017469268972No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1793500.25705276981633207No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT1348570.19328333080078666RNA PCR Primer, Index 33 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1033830.14817332869764066No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT911000.130568761250448RNA PCR Primer, Index 33 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT775770.11118696807383101No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)