FastQCFastQC Report
Sat 25 May 2024
EGAF00004973874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73303164
Total Bases9.2 Gbp
Sequences flagged as poor quality0
Sequence length126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1808400.24670149299421784No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT1443000.1968537128902103No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT993910.1355889631176084No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT822700.11223253610171588TruSeq Adapter, Index 1 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT818120.11160773360342263No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)