FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004974831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19148416
Sequences flagged as poor quality0
Sequence length150
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT8059654.209042669639097No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGC3607071.8837432819508413No Hit
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCA2948651.5398923858767222No Hit
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAG2693241.406507984785791No Hit
TACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGA1385380.7234958755857404No Hit
AGCAGCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1366720.7137509442034266Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG966750.5048720479020301No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC868080.4533429814769013No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT770480.4023727080088505No Hit
CCGACTGTTTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA741070.3870137352353323TruSeq Adapter, Index 2 (100% over 36bp)
AATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAA714620.3732005822309271No Hit
CAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA621620.32463259624190327TruSeq Adapter, Index 2 (100% over 40bp)
TCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAG600840.3137805236736031No Hit
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCACA575900.30075594764600894No Hit
CAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG572510.2989855662212478TruSeq Adapter, Index 2 (100% over 38bp)
AGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG571760.29859388891488464TruSeq Adapter, Index 2 (100% over 35bp)
CCCACTAATAGGGAACGTGAGCAGATCGGAAGAGCACACGTCTGAACTCC554070.28935552684880045Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT547050.28568942726124186No Hit
ACAGCCTCTGGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTC541710.28290068483993663No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTAGATCGGAAGAGCACAC537990.2809579654003757No Hit
ACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCAC510220.2664554603367715No Hit
ACAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT494410.2581989027186374TruSeq Adapter, Index 2 (100% over 37bp)
TCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACG452280.2361970828292011No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCAC431780.22549123645527652No Hit
GCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTC426720.2228487202283468Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG407420.21276955754460317No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC386100.2016354773157216No Hit
GCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG383950.20051266903748072TruSeq Adapter, Index 2 (100% over 35bp)
CGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAGAGCACACG364630.19042306162556735No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC361810.18895035495364212No Hit
AGCAGCCGACTTAGAACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG356810.18633917291122148Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCC346380.18089224717073202Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGAGATCGGAAGAGCAC336100.1755236568915152No Hit
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCA334730.17480819301189193No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC333640.17423895532664424No Hit
TCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT325550.17001406278200767Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTAGATCGGAAGAG324670.16955449474254164No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG324150.16928293181012988Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT322860.16860924684318535No Hit
TCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCC303730.158618864348884Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA301120.1572558273227404Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA298410.15584056665574844Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAATGGATGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG294970.15404407341056303TruSeq Adapter, Index 2 (100% over 35bp)
TCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC288540.1506860933040101Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGACTGTTTAATTAAAACAAGATCGGAAGAGCACACGTCTGAACTCCAG285570.14913505117081224Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA282410.1474847841200024No Hit
CCCACTAATAGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTC281380.14694688061926375Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCA276980.14464904042193358No Hit
AGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA276630.14446625767896415TruSeq Adapter, Index 2 (100% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTC273520.1428421024485785TruSeq Adapter, Index 2 (100% over 50bp)
GTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGT262770.13722806105737415Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCACACGT259080.1353010087100677No Hit
AAGGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA254990.13316506179936763TruSeq Adapter, Index 2 (100% over 40bp)
CCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC253110.13218325735141748Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGT245080.12798969899128992Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT238850.1247361661664338TruSeq Adapter, Index 2 (100% over 39bp)
CCGACTGTTTAATTAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC238010.12429748758330714Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTCTGAACTCCA237100.12382225245158661Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAA236020.12325823713042375No Hit
AGCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC235780.12313290039238756Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACGTCTGA235710.12309634384379366Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGGTCTGTGATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG230710.12048516180137303TruSeq Adapter, Index 2 (100% over 35bp)
TCTGCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAA224250.11711151460256557Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGACTGTTTAATTAAAACAAAGCAAGATCGGAAGAGCACACGTCTGAAC222030.1159521497757308Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGA221760.11581114594544008No Hit
ACAGGTCTGTGATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC221470.11565969738697969Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA221080.11545602518767087TruSeq Adapter, Index 2 (100% over 36bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG217490.11358119648121287No Hit
ACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAA216940.11329396645654659Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGATGTAGCTCAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC216760.11319996390301945Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGCAATAACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC214750.11215026872196635Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA212370.11090734606977412Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC209620.10947119594644278Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC205630.10738747267659111Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAGA202260.1056275359799996No Hit
GACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA198970.10390937819608682TruSeq Adapter, Index 2 (100% over 40bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT198510.10366914944818413TruSeq Adapter, Index 2 (100% over 43bp)
GACGAAAAATAACAATACAGGACTCTAGATCGGAAGAGCACACGTCTGAA195410.10205021658188332Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTACTCTGATCGTTTTTTCACTGACCAGATCGGAAGAGCACACGTCTG194290.10146531180438109Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGTAATTCTAGAGCTAATACATGCCGACGAGATCGGAAGAGCACACG192190.10036861534656444No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGGT1018250.0138.255022
GCATTGG1026400.0137.10841
ATTGGTG1054000.0133.741183
TGGTTCA1298350.0126.621258
GTGGTTC1327450.0123.9322747
GGTGGTT1218000.0123.5835956
GTCCGAT255000.0123.4118966
GGTTCAG1348350.0122.326329
TCCGATG257650.0122.002857
CTGGTCC292700.0119.150723
GGTCCGA316250.0117.29025
TTGGTGG1233400.0115.58424
TGGTCCG320500.0115.3302154
TGGTGGT1370650.0114.878465
GCATTGT142450.0110.7933961
CATTTGT120750.0110.295812
GCTGGTC317800.0109.9165952
CCGATGG288300.0109.207198
GCATTTG125550.0105.801031
TCCCTGG146650.0105.15761
Produced by FastQC (version 0.11.5)