FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004974832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13597692
Sequences flagged as poor quality0
Sequence length150
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT6173534.5401307810178375No Hit
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCA1843461.3557153669902215No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGC1680021.2355184982863268No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG1132570.8329134091285492No Hit
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAG1064840.7831034855032751No Hit
AGCAGCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC853830.6279227386530009Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT833290.6128172339835319No Hit
AGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG769640.5660078195623198TruSeq Adapter, Index 2 (100% over 35bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT719920.5294427907324272No Hit
GCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTC655240.48187589482097404Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG605640.44539911626178913No Hit
TACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGA533510.3923533493772326No Hit
TGAGAGGTGTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA458110.336902762615891TruSeq Adapter, Index 22 (100% over 37bp)
CCGACTGTTTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGT410940.3022130520385371TruSeq Adapter, Index 12 (97% over 36bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC400190.2943072986209719No Hit
CAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGT377800.27784126894475913TruSeq Adapter, Index 22 (100% over 40bp)
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCACA377560.2776647684033437No Hit
GGGGATGTAGCTCAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC354200.2604853823722438Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCC348840.2565435369472996Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGT341700.2512926458401911TruSeq Adapter, Index 22 (100% over 40bp)
GCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG333510.2452695648643902TruSeq Adapter, Index 2 (100% over 35bp)
CCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCA332680.24465916715866193Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC330510.2430633080966976No Hit
CAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAC323240.23771681252965576TruSeq Adapter, Index 22 (100% over 38bp)
TCTGCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAA320560.23574588981718367Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGATCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG309170.2273694682891773TruSeq Adapter, Index 2 (100% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA299630.22035357176791473No Hit
TGAATGGATGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG297940.21911071378878122TruSeq Adapter, Index 2 (100% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC297230.21858856635376062No Hit
CCCACTAATAGGGAACGTGAGCAGATCGGAAGAGCACACGTCTGAACTCC295370.21722068715779116Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGT291020.2140216148446369TruSeq Adapter, Index 12 (97% over 36bp)
CCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACGTCTGA285150.20970470576918496Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCAC283250.20830740981631293No Hit
ACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCAC283060.20816768022102577No Hit
CCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGT275490.20260055897721466TruSeq Adapter, Index 12 (97% over 36bp)
AGACGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGT266760.19618035178322907TruSeq Adapter, Index 22 (100% over 40bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCG262280.19288567501014142TruSeq Adapter, Index 22 (97% over 36bp)
AATAGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA261980.19266504933337217Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGACTGTTTAATTAAAACAAGATCGGAAGAGCACACGTCTGAACTCCAG261460.19228263149363878Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGCGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGT254590.18723030349562264TruSeq Adapter, Index 22 (100% over 40bp)
AATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAA244740.17998642710836515No Hit
TCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAG236580.17398540870024118No Hit
CCCACTAATAGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTC236040.17358828248205652Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA235670.17331617748070777TruSeq Adapter, Index 22 (100% over 37bp)
TCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACG228580.1681020573197275No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTAGATCGGAAGAGCACAC225820.16607230109345028No Hit
CACTACTCTGATCGTTTTTTCACTGACCAGATCGGAAGAGCACACGTCTG224780.1653074654139835Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGGTCTGTGATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG217970.1602992625513212TruSeq Adapter, Index 2 (100% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG217680.1600859910637776No Hit
CAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGT215860.15874752862471073TruSeq Adapter, Index 22 (100% over 40bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT214990.15810771416207986No Hit
TGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA212320.15614414563883341Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCCCGATCTCGTCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA211300.1553940183378179Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGT211220.1553351848240128TruSeq Adapter, Index 22 (100% over 40bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC204610.15047406574586333No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC201740.14836341343810405No Hit
TTGGAGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA200640.14755445262328343Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGAACTCCAGT198590.1460468438320268Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCACACGT191140.14056797285892342No Hit
AGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAA186720.13731742122118962TruSeq Adapter, Index 22 (97% over 40bp)
CCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC185690.13655993973094846Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAGCCTCTGGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTC184970.13603043810670223No Hit
TCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT182410.13414776566493786Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCA181540.133507951202307No Hit
GCAGGACGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA176850.13005883645548083TruSeq Adapter, Index 22 (100% over 37bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG176560.12984556496793723No Hit
ACCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA173390.12751428698340866Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC172260.1266832636009111Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG169670.12477852859146979Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGAGATCGGAAGAGCAC169220.12444759007631588No Hit
ACAGGTCTGTGATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC166940.1227708349328695Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC166500.12244725060694124Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCACTACTCTGATCGTTTTTTCACTGACCAGATCGGAAGAGCACACGTCT166220.12224133330862325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCA164070.12066018262511022No Hit
CGGGTGCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACG163120.1199615346486742TruSeq Adapter, Index 22 (100% over 39bp)
TCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCC161140.11850540518199706Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAGAGCACACG160760.11822594599142267No Hit
ACAGCCAAGGGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC160520.11804944545000726Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCAGCCGACTTAGAACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG160070.11771850693485335Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA155930.11467387259543751TruSeq Adapter, Index 22 (100% over 37bp)
GGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTCTGAACTCCA151390.11133507068699601Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCACTACTCTGATCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC148420.10915087648698028Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGT147120.10819483188764681Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTGACTGGCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA146080.10742999620818004TruSeq Adapter, Index 22 (100% over 37bp)
ACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACG144350.10615772147214396TruSeq Adapter, Index 22 (100% over 39bp)
GAGCAATAACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC142500.10479719646540016Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGCGACTCTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC142370.10470159200546682Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGAGGTGTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGT142150.1045397998425027TruSeq Adapter, Index 12 (97% over 36bp)
CCGACTGTTTAATTAAAACAAAGCAAGATCGGAAGAGCACACGTCTGAAC140630.10342196308020508Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTAGTTGGATCTTGGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCA139990.10295129496976398Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGGCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGT139720.10275273186067164TruSeq Adapter, Index 22 (100% over 40bp)
TTCTGCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGA137490.10111274766335346Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGGT812400.0139.126632
GCATTGG816900.0138.35281
ATTGGTG841400.0134.57263
TGGTTCA1061100.0132.608188
GTGGTTC1081700.0130.195957
GGTTCAG1091650.0129.121379
GTCCGAT222550.0129.015176
TCCGATG222500.0128.979457
GGTGGTT1007000.0126.1767356
CTGGTCC250500.0124.880693
GGTCCGA276850.0124.281295
CCGATGG234500.0122.440638
TGGTCCG284150.0121.0377354
CATTTGT109200.0120.643682
TGGTGGT1094000.0118.959275
TTGGTGG971200.0118.210564
TAGCTCA69100.0116.799458
GCTGGTC269050.0116.066452
GCATTTG113750.0115.932111
TCCCTGG142150.0113.306681
Produced by FastQC (version 0.11.5)