FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004974833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19032633
Sequences flagged as poor quality0
Sequence length150
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT5668062.9780745522703036No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGC3970052.0859173820038457No Hit
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCA3086111.6214834805042475No Hit
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAG2441641.2828703206750216No Hit
AGCAGCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1354380.7116093711258973Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGA1232400.6475194472567195No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT920860.4838321634216348No Hit
CCCACTAATAGGGAACGTGAGCAGATCGGAAGAGCACACGTCTGAACTCC701270.36845663970928244Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG685210.3600185008558721No Hit
CCGACTGTTTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC645690.33925416415059334TruSeq Adapter, Index 1 (100% over 36bp)
TCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAG617040.3242010708660226No Hit
AATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAA611480.3212797724833973No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT558260.2933172724971895No Hit
CAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA549220.28856753555853254TruSeq Adapter, Index 1 (100% over 40bp)
AGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT546140.28694926235376894TruSeq Adapter, Index 1 (100% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG516050.2711395738046333No Hit
ACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCAC511630.2688172466731219No Hit
CCGACTGTTTAATTAAAACAAGATCGGAAGAGCACACGTCTGAACTCCAG459690.2415272758109716Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG453590.23832225420413453Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTAGATCGGAAGAGCACAC443260.23289473400763833No Hit
GCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTC440030.23119764879614924Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC430500.226190459302189TruSeq Adapter, Index 1 (100% over 38bp)
CGCGACCTCAGATCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCC428830.2253130189606451Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCACA425780.2237105081572266No Hit
CGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAGAGCACACG381480.20043469550429516No Hit
AAAGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGT372800.19587410738177952Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGAGATCGGAAGAGCAC361920.1901576098272898No Hit
TCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACG354290.1861487057518526No Hit
AGCAGCCGACTTAGAACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG341850.1796125633274177Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA333820.17539349390071252TruSeq Adapter, Index 1 (100% over 37bp)
CCCACTAATAGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTC331830.1743479212781542Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCACACGT326610.17160526344410676No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCAC326280.17143187702931065No Hit
GCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT321040.16867871092769982TruSeq Adapter, Index 1 (100% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA317510.16682400170275968No Hit
TGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA296810.15594794477464047Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAGCCTCTGGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTC293900.15441899184416577No Hit
CCGACTGTTTAATTAAAACAAAGCAAGATCGGAAGAGCACACGTCTGAAC290610.15269038182998643Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC285640.15007907734048148No Hit
CCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGAACTCCAGT283670.14904401298548656Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC279990.14711049175382093TruSeq Adapter, Index 1 (100% over 36bp)
TCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCC275400.14469884434802058Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC270630.14219262253414963Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA268390.14101569656704882Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAATGGATGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT265350.13941843989741198TruSeq Adapter, Index 1 (100% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC265190.1393343737569048No Hit
ACCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA264970.1392187828137074Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCA262190.13775813362239478Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC256870.13496293445053031TruSeq Adapter, Index 1 (100% over 36bp)
TGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTAGATCGGAAGAG255210.1340907482427681No Hit
TCTGCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAA254280.13360211380106998Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC248880.13076488155895194No Hit
TCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT247880.13023946818078194Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAA246490.1295091435851256No Hit
AAGGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA237480.12477516904781383TruSeq Adapter, Index 1 (100% over 40bp)
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCA234720.1233250281240646No Hit
TGAAAAGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCC225700.11858579945297112Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG224260.11782920418840631TruSeq Adapter, Index 1 (100% over 39bp)
CCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAGA222320.11680990223475649No Hit
GTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGT221840.11655770381323487Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAGAACTTTGAAGAGAGAGTAGATCGGAAGAGCACACGTCTGAACTCCA221530.11639482566600218Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT219870.11552263945823996No Hit
TTGGAGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA218390.11474502765854834Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CACTACTCTGATCGTTTTTTCACTGACCAGATCGGAAGAGCACACGTCTG218040.11456113297618885Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC217290.11416707294256134No Hit
CCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACGTCTGA214200.112543545604016Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCA212910.11186576234617669No Hit
CAGGTCTGTGATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT207960.10926496612423514TruSeq Adapter, Index 1 (100% over 35bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC207460.10900225943515013No Hit
CCACTACTCTGATCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA207130.10882887302035404Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGAAAAATAACAATACAGGACTCTAGATCGGAAGAGCACACGTCTGAA206390.10844006712050823Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGACTGGCTCAGCGTGTGCAGATCGGAAGAGCACACGTCTGAACTCCAG205640.10804600708688072Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAA203620.1069846720629773Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTCTGAACTCCA200080.10512470870425547Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACAGCCAAGGGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA199790.10497233882458618Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGACTGTTTAATTAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC192860.10133122411386801Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGA192730.10126292037470591No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGGT761950.0138.587132
GCATTGG769850.0137.139271
ATTGGTG791400.0133.421683
TGGTTCA992350.0133.378868
GTGGTTC1016900.0130.229637
GGTTCAG1037350.0127.988539
GGTGGTT943200.0122.391146
CATTTGT138800.0119.3637162
GTCCGAT176200.0118.373496
TCCGATG177300.0117.6796957
GCATTTG145750.0113.763431
TGGTGGT1061800.0113.188835
TTGGTGG948600.0112.715724
GACCTCA184050.0112.6988144
CTGGTCC197550.0111.046843
GGTCCGA217750.0108.91245
TGGTCCG218200.0108.2258454
CCTCAGA191650.0107.9667056
ACCTCAG192400.0107.920045
CCGATGG195600.0106.780218
Produced by FastQC (version 0.11.5)