FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004974835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11185937
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGC2410422.1548664184323583No Hit
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCA1899601.6982037356369877No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1890441.6900148820791678No Hit
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAG1863861.666252903087153No Hit
TACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGA1037720.9277005582992288No Hit
AGCAGCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC649360.5805146229591673Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAA587890.5255616941164607No Hit
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCACA396300.3542841337297001No Hit
TCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAG378000.33792430620698116No Hit
AGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC350620.31344714349812625TruSeq Adapter, Index 5 (100% over 35bp)
ACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCAC321580.28748597457682806No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCAC315430.2819879997536192No Hit
ACAGCCTCTGGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTC314130.2808258262137539No Hit
CCCACTAATAGGGAACGTGAGCAGATCGGAAGAGCACACGTCTGAACTCC295800.2644391793016535Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTAGATCGGAAGAGCACAC294100.26291941390336815No Hit
CAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA283940.25383658069949794TruSeq Adapter, Index 8 (100% over 40bp)
CAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG280420.2506897723454012TruSeq Adapter, Index 8 (100% over 38bp)
CCGACTGTTTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT274370.2452811954867974TruSeq Adapter, Index 8 (100% over 36bp)
ACAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT264580.2365291347519658TruSeq Adapter, Index 8 (100% over 37bp)
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCA253640.22674899742417648No Hit
TCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGA241540.21593184370696886No Hit
TCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACG239530.2141349446184079No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTC226740.20270094494542565TruSeq Adapter, Index 8 (100% over 50bp)
TGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTAGATCGGAAGAG222440.19885683246740976No Hit
CGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAGAGCACACG219180.19594245882128603No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG211070.1886922838918188No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC209890.18763738790947956No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC208860.18671658887404785No Hit
GCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTC207430.18543819798019603Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA188250.1682916683689529Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT184620.16504652225379063Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCAGCCGACTTAGAACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG177240.15844895246594004Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA173560.15515910736847524TruSeq Adapter, Index 8 (100% over 40bp)
ACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGAGATCGGAAGAGCAC170320.15226261331527255No Hit
GATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCA166510.14885655086382124No Hit
TGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA163560.1462193109079731Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC159880.14292946581050833Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT157280.14060511873077775TruSeq Adapter, Index 8 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG157120.14046208198740973No Hit
CGCGACCTCAGATCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCC156900.14026540646527869Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCC155510.13902277475726887Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC154180.1378337818280221No Hit
CTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCACACGT152280.13613522050052668No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG150330.13439196019072877Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA148850.13306887031457446Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAA146680.13112893448264548No Hit
TCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA146580.13103953651804046Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGT146380.1308607405888304Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGC145390.12997570073924072No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA144560.12923369763301903No Hit
GACGAAAAATAACAATACAGGACTCTAGATCGGAAGAGCACACGTCTGAA143620.12839335676573183Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT142900.12774969142057568No Hit
TGAATGGATGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC142070.127007688314354TruSeq Adapter, Index 5 (100% over 35bp)
CGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGC140540.12563989945589718No Hit
GCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC139490.12470122082754445TruSeq Adapter, Index 5 (100% over 35bp)
CCCACTAATAGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTC133310.1191764266149541Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCG131780.11780863775649729No Hit
GTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGT131760.11779075816357627Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAGA131480.11754044386268223No Hit
GGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTC131030.11713815302195962No Hit
CACTACTCTGATCGTTTTTTCACTGACCAGATCGGAAGAGCACACGTCTG130830.11695935709274959Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA129220.11552004986260873TruSeq Adapter, Index 8 (100% over 39bp)
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA128880.11521609678295165No Hit
TGTGGTAATTCTAGAGCTAATACATGCCGACGAGATCGGAAGAGCACACG127150.11366951199528479No Hit
GGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTCTGAACTCCA124180.11101439244651566Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC118880.10627630032244952Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCTGCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAA118720.10613326357908148Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGC117660.10518564515426825No Hit
GAGCAATAACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC117640.10516776556134726Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACGTCTGA116940.1045419798091121Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGACTGTTTAATTAAAACAAGATCGGAAGAGCACACGTCTGAACTCCAG116700.10432742469406005Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTAGATCGGAAGAGC116010.1037105787382854No Hit
TGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGA115760.10348708382677285No Hit
ACAGGTCTGTGATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA112050.10017041933992656Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG111950.10008102137532154No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)