FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004974836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15904371
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT15157359.53030459362398No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG2621981.6485908182096605No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG2370231.4903009996434313No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1914341.203656529390568No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA1874121.1783678838980804No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1851091.163887587883859No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1824381.1470934625456108No Hit
TTGGATTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT1735221.091033402075442TruSeq Adapter, Index 10 (100% over 39bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC1679851.0562190733603989No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC1625081.021781999426447No Hit
TGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1517450.9541087792783506Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC1198330.7534595363752519No Hit
GGCATGTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT1153080.7250082383012821TruSeq Adapter, Index 10 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1076180.6766567505247457No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG1058490.6655340220622369No Hit
CAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA1054740.6631761796804162TruSeq Adapter, Index 10 (100% over 40bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT1048440.6592150044789574No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT644800.4054231380794625No Hit
AGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA643960.40489498138593477TruSeq Adapter, Index 7 (97% over 35bp)
AGACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA610200.38366811236986365TruSeq Adapter, Index 10 (100% over 40bp)
TGGATTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA600820.3777703626254694TruSeq Adapter, Index 10 (100% over 40bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA583450.366848836712876No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC557630.3506143059665799No Hit
CGTAGTTGGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG521120.32765835253717357TruSeq Adapter, Index 7 (97% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC513260.3227163149048774No Hit
TCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT464980.2923598801863966Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGGATTGTTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC418750.26329239930331105TruSeq Adapter, Index 10 (100% over 37bp)
ACAGCCAAGGGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT390030.2452344704484069Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGATGTAGCTCAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC363770.2287232861959772Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA360530.2266861103780841TruSeq Adapter, Index 10 (100% over 40bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCAC351050.22072548483684137No Hit
AGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATC334980.21062134428327908TruSeq Adapter, Index 10 (100% over 42bp)
AGCCTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA333580.20974108312739936TruSeq Adapter, Index 10 (100% over 40bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT331750.20859045604507087No Hit
GTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC326560.20532720218863104Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT313710.1972476622935921Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT304720.19159512815690732TruSeq Adapter, Index 10 (100% over 39bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA303860.19105439630400975No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA294900.1854207249063795No Hit
TCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC292860.18413805865066907Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTCTGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA277270.17433572192197982TruSeq Adapter, Index 10 (100% over 40bp)
CTCTGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT276480.17383900312687625TruSeq Adapter, Index 10 (100% over 41bp)
CAGCCAAGGGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA275230.17305305566626936TruSeq Adapter, Index 7 (97% over 35bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGAGATCGGAAGAGCACACG265070.1666648747064565No Hit
GGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA260870.1640240912388173TruSeq Adapter, Index 7 (97% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC251110.15788741346639865No Hit
TCTGTGGGATTATGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA242010.15216571595318043Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATC238990.15026686688835414TruSeq Adapter, Index 10 (100% over 42bp)
GGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC232290.1460541884995012Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC224570.14120017698279297No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA219520.13802494924194111No Hit
CGGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATC217780.1369309103767763TruSeq Adapter, Index 10 (100% over 42bp)
GGCATGTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT213760.1344033033434645TruSeq Adapter, Index 10 (100% over 39bp)
CACAGCCAAGGGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC204140.12835465168663382Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT202630.12740522715422067TruSeq Adapter, Index 10 (100% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTC199390.1253680513363276TruSeq Adapter, Index 10 (100% over 50bp)
AGCTCGTAGTTGGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA198810.125003371714606Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAGCAGTTGAACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA198060.12453180323824187TruSeq Adapter, Index 7 (97% over 35bp)
CGCGGACGCTACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG191980.12070895478984991TruSeq Adapter, Index 7 (97% over 36bp)
ATCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAG190440.11974066751838221Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC189170.1189421448984056Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT188150.11830081177055037TruSeq Adapter, Index 10 (100% over 41bp)
CAGGGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT186990.11757145252710717TruSeq Adapter, Index 10 (100% over 41bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAGATCGGAAGAGCACACGTCT185790.11681694296492456Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT181590.11417615949728538TruSeq Adapter, Index 10 (100% over 41bp)
GGGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA178530.11225216011371969Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACTC173220.10891345530106157Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)