FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004974837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15167793
Sequences flagged as poor quality0
Sequence length150
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGC2237401.4750992448275106No Hit
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCA1713651.1297952180650144No Hit
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAG1696211.118297170854059No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1688701.1133458902030111No Hit
TACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGA934670.6162201712536557No Hit
AGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC614360.4050424475070302TruSeq Adapter, Index 5 (97% over 35bp)
AGCAGCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC604410.39848249511316514Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAA602590.397282584223031No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC511770.33740571222194293No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG372140.24534881244753273No Hit
AGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC358610.23642859577527198TruSeq Adapter, Index 6 (100% over 36bp)
CCCACTAATAGGGAACGTGAGCAGATCGGAAGAGCACACGTCTGAACTCC338760.2233416555724356Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAG320890.21156011293139348No Hit
TGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTAGATCGGAAGAG303870.20033896823354588No Hit
ACAGCCTCTGGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTC301560.19881600441145258No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG297110.19588215635590492No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT296750.1956448113446696No Hit
CAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA295360.19472839588462212TruSeq Adapter, Index 6 (100% over 40bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC292490.19283622871171832No Hit
GCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTC289950.19116162779911355Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCAC284510.18757508096266873No Hit
TCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACG282450.18621694006504438No Hit
TCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT278860.18385008286966997Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG278180.18340176451511436No Hit
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCACA272050.17936030640713516No Hit
TCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCC265330.17492986619740922Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA264040.1740793799071493No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCAC260520.1717586731306262No Hit
CAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA246180.16230443018308596TruSeq Adapter, Index 6 (100% over 38bp)
TCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG245760.1620275276699781Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCA244930.1614803155607411No Hit
AAGGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA240310.1584343879165545TruSeq Adapter, Index 6 (100% over 40bp)
GTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGT235520.155276380683729Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC235290.15512474359321754TruSeq Adapter, Index 6 (100% over 50bp)
TGTGGTAATTCTAGAGCTAATACATGCCGACGAGATCGGAAGAGCACACG231670.15273810764690685No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTAGATCGGAAGAGCACAC229760.1514788605039639No Hit
GACGAAAAATAACAATACAGGACTCTAGATCGGAAGAGCACACGTCTGAA227100.14972514458761402Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGAGATCGGAAGAGCAC222500.14669240277738496No Hit
AGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA215920.14235426340536161Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA215770.14225536965068022Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA215190.1418729804659122TruSeq Adapter, Index 6 (100% over 37bp)
GGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTC210840.1390050615801521No Hit
GAGCAATAACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC209990.13844466363695762Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC208300.13733046066754734Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC206340.1360382489397106Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAGAGCACACG205940.13577453226056024No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA205790.13567563850587885No Hit
CCGACTGTTTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC195970.12920139403273767TruSeq Adapter, Index 6 (100% over 36bp)
CGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGC194550.12826519982175388No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC188090.12400617545347567No Hit
GGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTCTGAACTCCA187400.12355126418194129Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCA185880.12254914080116995No Hit
TGAATGGATGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC184600.12170524742788882TruSeq Adapter, Index 5 (97% over 35bp)
AAAGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGT182290.12018228360579553Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GACGAAAAATAACAATACAGGACTAGATCGGAAGAGCACACGTCTGAACT179990.11866591270068098Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA179760.11851427561016953Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGA178530.11770334682178218No Hit
ACCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA172160.11350365870631278Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC171330.11295644659707578Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAG169340.11164445611830279Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTGCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAA169170.11153237652966387Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC165250.1089479530739904TruSeq Adapter, Index 6 (100% over 36bp)
CCCACTAATAGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTC160450.10578335292418613Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGAGGTGTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA158250.10433291118885919TruSeq Adapter, Index 6 (100% over 37bp)
CCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC157930.10412193784553889No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC156670.10329123030621529No Hit
CCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC155310.10239459359710408Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGC155020.10220339900472006No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTGG300300.0127.192361
CATTGGT304500.0125.651542
ATTGGTG315700.0121.1884163
GTCCGAT189900.0120.411966
TGGTTCA365000.0119.495228
TCCGATG193450.0118.313947
CTGGTCC219200.0118.04613
GTGGTTC381800.0114.2371757
TCCCTGG184400.0113.891461
GGTCCGA257300.0113.829615
TGGTCCG261300.0111.949344
CCCTGGT187050.0111.9259642
CCGATGG208250.0110.009278
GCTGGTC238900.0108.2075652
GGTTCAG409250.0106.733179
GGTGGTT394900.0102.772056
CCTGGTG208200.0100.595173
TGGTGGT621550.096.385925
GATCGAT69850.095.154311
TCCCTGT118400.093.601041
Produced by FastQC (version 0.11.5)