FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004974838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15407950
Sequences flagged as poor quality0
Sequence length150
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2774301.800563994561249No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGC2744951.7815153865374693No Hit
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCA2149831.3952732193445592No Hit
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAG2066381.3411128670588885No Hit
TACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGA1090010.7074335002385133No Hit
AGCAGCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1000780.6495218377525888Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAA576850.3743846520789592No Hit
CCCACTAATAGGGAACGTGAGCAGATCGGAAGAGCACACGTCTGAACTCC533630.34633419760578144Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC493190.3200880065161167TruSeq Adapter, Index 5 (100% over 35bp)
CAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT479490.31119649271966743TruSeq Adapter, Index 5 (100% over 38bp)
CAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA460930.29915076308009825TruSeq Adapter, Index 5 (100% over 40bp)
TCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAG447460.2904085228729325No Hit
CCGACTGTTTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA441250.28637813596227923TruSeq Adapter, Index 5 (100% over 36bp)
ACAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG438730.284742616636217TruSeq Adapter, Index 5 (100% over 37bp)
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCACA430080.279128631647948No Hit
ACAGCCTCTGGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTC424900.27576673081104236No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTAGATCGGAAGAGCACAC421300.2734302746309535No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG415840.2698866494244854No Hit
ACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCAC368630.23924662268504246No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCAC344540.22361183674661456No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC334020.2167841925759105No Hit
TCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACG314230.2039401737414776No Hit
GCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTC297340.19297830016322742Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC294470.19111562537521215TruSeq Adapter, Index 5 (100% over 50bp)
CGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAGAGCACACG276960.1797513621215022No Hit
AAGGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA274370.17807041170304938TruSeq Adapter, Index 5 (100% over 40bp)
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCA274000.17783027592898473No Hit
TCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCC272260.1767009887752751Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG269070.17463062899347415Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT266630.17304703091585838Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCAGCCGACTTAGAACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG266320.1728458360781285Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGAGATCGGAAGAGCAC258840.16799119934838833No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC257520.16713449874902242No Hit
ACCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA250870.16281854497191386Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTAGATCGGAAGAG250580.1626303304462956No Hit
GTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGT240170.15587407799220532Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT238250.15462796802949128No Hit
CCCACTAATAGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTC237200.15394650164363202Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCC237150.153914050863353Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG234700.15232396262968143No Hit
TCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA234170.1519799843587239Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA232540.15092208892162812TruSeq Adapter, Index 5 (100% over 36bp)
GCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC217190.1409596993759715TruSeq Adapter, Index 5 (100% over 35bp)
TGAATGGATGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC216820.14071956360190682TruSeq Adapter, Index 5 (100% over 35bp)
TGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA214270.1390645738076772Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC211480.13725382026810834Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA205290.13323641366956668Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT203060.13178910886912276No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC202840.1316463254358951No Hit
GATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCA201820.13098432951820327No Hit
ACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG199810.12967980815098698TruSeq Adapter, Index 5 (100% over 39bp)
AGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA199320.12936179050425267Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG198840.12905026301357417No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC196880.1277781924266369No Hit
GGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTCTGAACTCCA194280.12609075185212829Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA193530.1256039901479431No Hit
CTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCACACGT193040.1252859725012088No Hit
GAGCAATAACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC190880.12388409879315548Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAAGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGT189100.12272885101522267Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT184820.11995106422333926No Hit
GACGAAAAATAACAATACAGGACTCTAGATCGGAAGAGCACACGTCTGAA181280.11765354897958522Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC181260.1176405686674736Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC176700.11468105750602772Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACAGGTCTGTGATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA174900.1135128294159833Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAA173810.11280540240590085Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAGA172350.11185783962175372No Hit
TCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGA171290.11116988307983866No Hit
CCGACTGTTTAATTAAAACAAGATCGGAAGAGCACACGTCTGAACTCCAG171050.1110141193344994Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGGTCTGTGATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC168710.10949542281744164TruSeq Adapter, Index 5 (100% over 35bp)
CGGCGACTCTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA168140.10912548392226092Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTC164760.10693181117539972No Hit
CCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA160090.10390090829734001TruSeq Adapter, Index 5 (100% over 36bp)
ACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCC158460.10284301286024422Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGTAATTCTAGAGCTAATACATGCCGACGAGATCGGAAGAGCACACG158020.10255744599378892No Hit
GGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC157530.10223942834705461Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAA157070.1019408811684877No Hit
CTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATC154600.10033781262270451TruSeq Adapter, Index 5 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGGT391100.0133.247742
GCATTGG397250.0131.22131
ATTGGTG414050.0125.979323
TGGTTCA480750.0124.270428
GTGGTTC501200.0119.300487
GGTTCAG524500.0114.261579
GTCCGAT142350.0113.9002156
TCCGATG145200.0111.714157
CTGGTCC170050.0111.011943
GGTGGTT512350.0108.497686
GGTCCGA198350.0106.570615
TGGTCCG199950.0105.53784
CCGATGG156550.0103.5228048
TCCCTGG115950.0102.764241
GACCTCA117900.0100.8200454
TGGTGGT654500.098.782565
GCTGGTC192950.098.308362
CCCTGGT121850.097.649692
TAGCTCA39050.097.3478858
ACCTCAG121850.097.315425
Produced by FastQC (version 0.11.5)