FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004974839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16328638
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGC2026461.2410465588128048No Hit
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCA1603810.9822068441960683No Hit
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAG1470570.9006078767867841No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1160110.7104756685768893No Hit
AGCAGCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC743810.4555248269941437Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGA712360.4362641881092593No Hit
CAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAC601670.3684753131277697TruSeq Adapter, Index 13 (100% over 40bp)
AGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG571300.349876088869139TruSeq Adapter, Index 2 (97% over 35bp)
CAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCA546350.33459618616078085TruSeq Adapter, Index 13 (100% over 38bp)
CCCACTAATAGGGAACGTGAGCAGATCGGAAGAGCACACGTCTGAACTCC523800.32078609373298617Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC511700.31337580023514516No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCG495250.30330147560378273TruSeq Adapter, Index 13 (97% over 36bp)
ACAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTC488800.2993513604747683TruSeq Adapter, Index 13 (100% over 37bp)
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTAGATCGGAAGAGCACAC466750.2858474785220911No Hit
TCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT439920.26941622442729146Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGACTGTTTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT379870.232640346365692TruSeq Adapter, Index 8 (97% over 36bp)
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCACA347260.2126692991785353No Hit
AGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT336100.20583468137391495TruSeq Adapter, Index 8 (97% over 36bp)
ACAGCCTCTGGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTC329820.20198867780644045No Hit
AATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAA323990.1984182636665716No Hit
ACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAA312720.19151627955742542TruSeq Adapter, Index 13 (100% over 39bp)
ACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCAC307080.1880622253981012No Hit
TCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA306200.18752329496189457Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA292280.1789983953346262Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA288490.17667731993323632Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAC288330.17657933258119876TruSeq Adapter, Index 13 (100% over 40bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG280340.17168608918882272No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG276260.16918741171186474Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTC273740.16764411091727308Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC273570.16753999935573316Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACG272850.1670990562715641No Hit
TCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCC266230.16304482958100977Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGAGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA262710.16088910783618326Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCAC260220.15936417967009864No Hit
TCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAG253590.15530382877004192No Hit
CGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAGAGCACACG239020.14638085552512095No Hit
CCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC236050.14456196530292362Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGT235330.14412102221875456Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAA231020.14148148792324258TruSeq Adapter, Index 13 (97% over 40bp)
ATCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAG230700.14128551321916744Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC229960.14083232171599372Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGCAATAACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC225830.138303023191524Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG220200.1348550932417021No Hit
CCCACTAATAGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTC209370.12822257435065926Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCC206570.12650779569000184Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAATGGATGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG200890.12302924469266818TruSeq Adapter, Index 2 (97% over 35bp)
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCA197910.12120423026096849No Hit
CCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT196970.12062855456774779TruSeq Adapter, Index 8 (97% over 36bp)
AGCAGCCGACTTAGAACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG191770.11744396562652684Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT191070.11701527096136248No Hit
CGGCGACTCTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA190580.11671518469574743Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAC189460.11602927323148447TruSeq Adapter, Index 13 (100% over 40bp)
GCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG187150.11461458083644209TruSeq Adapter, Index 2 (97% over 35bp)
AGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAA186230.11405115356222609TruSeq Adapter, Index 13 (97% over 40bp)
TTGGATTGTTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTC185900.11384905464864858TruSeq Adapter, Index 13 (100% over 37bp)
CCGACTGTTTAATTAAAACAAGATCGGAAGAGCACACGTCTGAACTCCAG182010.11146673715223523Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA181250.11100129723005678Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAGGTCTGTGATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA179350.10983769742461068Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTCTGAACTCCA176240.10793306826938046Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGGTCTGTGATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG176030.10780445986983114TruSeq Adapter, Index 2 (97% over 35bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAAT175380.10740638625217855TruSeq Adapter, Index 13 (97% over 40bp)
CCGACTGTTTAATTAAAACAAAGCAAGATCGGAAGAGCACACGTCTGAAC172510.10564873812500468Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGAGATCGGAAGAGCAC166910.1022191808036898No Hit
GATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCA166480.10195583979508885No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA166420.10191909453807475No Hit
AGCCTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAC165860.10157613880594327TruSeq Adapter, Index 13 (100% over 40bp)
ATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT164940.10101271153172726Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)