FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004974840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19915969
Sequences flagged as poor quality0
Sequence length150
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCA2993721.5031756677267372No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGC1935940.9720541340469048No Hit
AGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA1681560.8443274841409927TruSeq Adapter, Index 7 (97% over 35bp)
AGCAGCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1462300.7342349247480753Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1431630.7188352221275299No Hit
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAG1338980.6723147640970922No Hit
GCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTC1197110.6010804696472464Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCACA899920.4518585061063311No Hit
GTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC790590.3969628593014982Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGA774850.3890596535875307TruSeq Adapter, Index 7 (97% over 36bp)
TCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG761630.38242176416321993Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGAGGTGTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT748520.37583910679917204TruSeq Adapter, Index 7 (97% over 36bp)
CAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTG728970.3660228633615567TruSeq Adapter, Index 7 (97% over 36bp)
CCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCA711350.3571756915267342Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA704900.35393708435677923TruSeq Adapter, Index 7 (97% over 35bp)
TACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGA680710.3417910521953514No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC677910.3403851452068438No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCAC638800.32074763723522565No Hit
AGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG635460.31907059104179164TruSeq Adapter, Index 7 (97% over 36bp)
TGCAGGACACATTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA592460.2974798765754255Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCG569710.2860568822938015TruSeq Adapter, Index 7 (96% over 32bp)
CGGGCGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGA566570.28448025802811805TruSeq Adapter, Index 7 (97% over 36bp)
GTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGT527620.2649230875987003Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC525980.26409962779114593Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCAC507870.2550064222333345No Hit
ACCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA500080.25109498814745096Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAATGGATGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA496390.24924220358045346TruSeq Adapter, Index 7 (97% over 35bp)
TCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT486560.2443064658315144Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA484600.24332233093955913Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGACTGTTTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG470140.23606182556319505TruSeq Adapter, Index 7 (97% over 36bp)
TCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCC451670.22678786053543265Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAATCCGACTGTTTAATTAGATCGGAAGAGCACACGTCTGAACTCCA449080.22548739657106315Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTGCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAA436130.2189850767492157Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTACTCTGATCGTTTTTTCACTGACCAGATCGGAAGAGCACACGTCTG435580.2187089164479017Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT423220.2125028413129183TruSeq Adapter, Index 7 (97% over 36bp)
ATCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAG413970.2078583271544558Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG406780.2042481588518239TruSeq Adapter, Index 7 (97% over 36bp)
ACTGGCTTGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT406220.20396697745412237TruSeq Adapter, Index 7 (97% over 36bp)
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCA395000.19833330730731707No Hit
AATAGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA391800.1967265564633084Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGCGACTCTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG376190.18888862500237874Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACGTCTGA372000.18678478561600492Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG354530.17801293022699521No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA354450.17797276145589502No Hit
CAGGTCTGTGATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA352180.17683297257592637TruSeq Adapter, Index 7 (97% over 35bp)
CCACTACTCTGATCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG350370.17592415412978402Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTGCAGGACACATTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT349490.1754822976476816Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGAACGTGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG336810.16911554742829735TruSeq Adapter, Index 7 (97% over 36bp)
CGTGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGA334540.16797575854832875TruSeq Adapter, Index 7 (97% over 36bp)
CGGGCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGAT331220.16630875454766977TruSeq Adapter, Index 7 (97% over 36bp)
AGGGGAATCCGACTGTTTAATTAGATCGGAAGAGCACACGTCTGAACTCC329380.16538487281236477Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG325980.1636777000406056Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCCCGATCTCGTCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA321000.16117719403961714Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC318000.15967086512335907No Hit
TTGGATTGTTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT317390.1593645782437199TruSeq Adapter, Index 7 (97% over 36bp)
TCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC313140.15723061227902094Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCACTACTCTGATCGTTTTTTCACTGACCAGATCGGAAGAGCACACGTCT311250.1562816250617783Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGTGGGATTATGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA310280.15579457871218821Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA310000.15565398801333744No Hit
AATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAA305380.15333424148229996No Hit
CCCGTCTATGTAGCAAAATAGATCGGAAGAGCACACGTCTGAACTCCAGT304410.15284719513270983Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCACACGT301610.15144128814420227No Hit
CTGGCTTGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTG300930.15109985358985042TruSeq Adapter, Index 7 (97% over 36bp)
TCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACG294620.14793154176932088No Hit
GGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC293310.14727377814255485Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA292570.14690221700987785Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGA291830.14653065587720085TruSeq Adapter, Index 7 (97% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA291220.1462243689975617No Hit
AAGGGCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGA290390.14580761799739694TruSeq Adapter, Index 7 (97% over 36bp)
ATCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC286840.14402512877982487Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAGCCAAGGGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG286600.14390462246652422Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGA280410.140796563802645TruSeq Adapter, Index 7 (97% over 36bp)
CAGATCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA269880.13550934930657907TruSeq Adapter, Index 7 (97% over 35bp)
CCCACTAATAGGGAACGTGAGCAGATCGGAAGAGCACACGTCTGAACTCC261110.13110584777471784Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGTGCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGG260900.13100040475057978TruSeq Adapter, Index 7 (97% over 36bp)
ACCATCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTC255890.12848483546042877Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTACTCTGATCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA250490.12577344341116417TruSeq Adapter, Index 7 (97% over 35bp)
AGCAGCCGACTTAGAACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG248840.12494496250722222Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCACTACTCTGATCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC245610.12332314837405099Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAGGTCTGTGATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG244820.12292648175943637Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGACGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGA242870.12194736796386858TruSeq Adapter, Index 7 (97% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG237240.11912049069769089No Hit
ATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT237140.1190702797338156Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATAT230990.11598230545548649TruSeq Adapter, Index 7 (97% over 36bp)
CGTAGTTGGATCTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC230480.11572622953972263Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAGGACGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT227060.11400901457518837TruSeq Adapter, Index 7 (97% over 36bp)
TCTGGCATGTTGGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC227040.11399897238241333Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGCGTGGAATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG226480.11371779098471181TruSeq Adapter, Index 7 (97% over 36bp)
GGGGATGTAGCTCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT222630.11178466887551392Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCCAGTGCTCTGAATGTCAAAGTAGATCGGAAGAGCACACGTCTGAACT222610.11177462668273887Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGGCATGTTGGAACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA221700.1113177069114739Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG220340.11063483780277023Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAACTATGCCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC219060.10999213746516678Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGACTGTTTAATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA218470.10969589277830268TruSeq Adapter, Index 7 (97% over 35bp)
AGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATA217790.10935445822395085TruSeq Adapter, Index 7 (97% over 36bp)
ACAGCCTCTGGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTC216790.10885234858519814No Hit
TAGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG216350.10863142034414695Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGA214790.10784812930769273TruSeq Adapter, Index 7 (97% over 36bp)
CTGACTGGCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT214680.10779289724742994TruSeq Adapter, Index 7 (97% over 36bp)
ACCACTACTCTGATCGTTTTTTCACTGACCAGATCGGAAGAGCACACGTC214180.10754184242805359No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG213910.10740627282559037No Hit
GGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA207150.10401201166762211Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGT205610.10323876282394293Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGAGGTGTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG205410.1031383408961924TruSeq Adapter, Index 7 (97% over 36bp)
CAGGTCTGTGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG203380.1021190583295244TruSeq Adapter, Index 7 (97% over 36bp)
AGACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGA202130.10149142128108354TruSeq Adapter, Index 7 (97% over 36bp)
ACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAA200020.10043196994331534Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACT199920.10038175897944007Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGGT254350.0122.770392
TGGTTCA312850.0120.404838
TCCCTGG239450.0118.427071
GCATTGG266600.0116.908511
TCCGATG175600.0115.2930457
GTCCGAT177600.0113.9541556
CCCTGGT250750.0112.778082
CTGGTCC211650.0109.1600653
GGTCCGA236050.0108.6126255
GTGGTTC349750.0107.825167
TAGCTCA75100.0106.988378
GGTTCAG356400.0106.035489
ATTGGTG295000.0105.822883
TGGTCCG246150.0104.1560444
CCGATGG194950.0104.071088
CCTGGTG273600.0103.4162453
TCCCTGT138850.0101.932971
GCTGGTC241200.095.880232
CAGCCGA379700.091.887223
GTAGCTC81300.090.770697
Produced by FastQC (version 0.11.5)