FastQCFastQC Report
Fri 1 Jul 2022
EGAF00004974841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30981098
Sequences flagged as poor quality0
Sequence length150
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT25379688.191988547339413No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG3849611.2425673228237424No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3439431.1101704658756768No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG3215381.0378521768337585No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3145651.015344904819061No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2031210.6556287966294804No Hit
AGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC1943510.6273212137284483TruSeq Adapter, Index 6 (97% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC1853270.5981937760888913No Hit
GTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1829390.5904858504369341Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG1690410.5456262395864729TruSeq Adapter, Index 2 (97% over 35bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC1667780.538321785754656No Hit
CAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA1660930.5361107601802879TruSeq Adapter, Index 11 (100% over 40bp)
TCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT1581680.5105306467834032Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1549980.5002986014246493Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTA1498830.4837885345445148TruSeq Adapter, Index 11 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA1480450.47785588490117425No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT1315030.4244620381111089No Hit
ACAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT1232120.3977005592248538TruSeq Adapter, Index 11 (100% over 37bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG1221240.3941887405023541No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTC1053800.3401428832509422TruSeq Adapter, Index 11 (100% over 50bp)
TCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1041450.3361565816679577Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGATGTAGCTCAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1028150.3318636415016666Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC1002970.32373610515676365No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG998910.32242562868494845No Hit
ATCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAG937790.3026974705673763Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGATTGTTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT918160.29636134910389555TruSeq Adapter, Index 11 (100% over 37bp)
TCTGTGGGATTATGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA890090.2873009859108286Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC876990.28307260123575995No Hit
AAGGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA820220.2647485250522754TruSeq Adapter, Index 11 (100% over 40bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC811820.2620371944209337No Hit
ACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTAC775720.2503849282552865TruSeq Adapter, Index 11 (100% over 39bp)
CCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC762320.24605971034338422TruSeq Adapter, Index 6 (97% over 36bp)
GGGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA761170.2456885162688553Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAGCCAAGGGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG697490.22513404786363606Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA688170.22212576197267123TruSeq Adapter, Index 11 (100% over 40bp)
CGTAGTTGGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC663770.21424999204353573TruSeq Adapter, Index 6 (97% over 36bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT649230.2095568078316656No Hit
GACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA613760.19810789146336907TruSeq Adapter, Index 11 (100% over 40bp)
CTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATC613330.19796909715724087TruSeq Adapter, Index 11 (100% over 42bp)
ATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT607920.19622287111967432Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGCGACTCTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG600590.19385691236637256Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGGACGCTACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC590850.1907130599438406TruSeq Adapter, Index 6 (97% over 36bp)
GGCATGTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTA578040.18657828073104446TruSeq Adapter, Index 11 (100% over 38bp)
GGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC575180.18565513720656382Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT566790.1829470343497832TruSeq Adapter, Index 11 (100% over 48bp)
TTGGATTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTAC558180.18016792045265792TruSeq Adapter, Index 11 (100% over 39bp)
ATCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC540900.1745903260110407Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATC533450.17218563396300546TruSeq Adapter, Index 11 (100% over 42bp)
TGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA532880.17200165081302154Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCACTAATAGGGAACGTGAGCAGATCGGAAGAGCACACGTCTGAACTCC522000.16848983209052176Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC509070.1643163195829922No Hit
CGGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATC501200.16177606100338987TruSeq Adapter, Index 11 (100% over 42bp)
AGCCTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA487700.15741856534587637TruSeq Adapter, Index 11 (100% over 40bp)
GAGCAATAACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC486620.1570699656932753Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT480490.15509133988730806TruSeq Adapter, Index 11 (100% over 37bp)
CGGGCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT463930.1497461452140915TruSeq Adapter, Index 11 (100% over 41bp)
GTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG463320.14954925096586313Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA437730.1412893758639542TruSeq Adapter, Index 11 (100% over 40bp)
TTGGAGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA418230.13499521546976803Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAGCAGTTGAACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG415380.13407529971984855TruSeq Adapter, Index 2 (97% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA412500.13314570064624565No Hit
CAGCCAAGGGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG407800.13162864660251872TruSeq Adapter, Index 2 (97% over 35bp)
GGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG400440.1292530045255336TruSeq Adapter, Index 2 (97% over 35bp)
CCCTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA399450.1289334548439826TruSeq Adapter, Index 11 (100% over 40bp)
CGACTCTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT381080.12300403297520314TruSeq Adapter, Index 11 (100% over 37bp)
GTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGT379730.12256828340945179Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT369860.11938246991762524TruSeq Adapter, Index 11 (100% over 41bp)
CCACTACTCTGATCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG363780.11741998298446363Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGTGGGATTATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG356510.11507339087852858TruSeq Adapter, Index 2 (97% over 35bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCT355620.11478611894258879TruSeq Adapter, Index 11 (100% over 43bp)
AGCTCGTAGTTGGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA355350.11469896902943855Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGGGATTATGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG353580.11412765293212009TruSeq Adapter, Index 2 (97% over 35bp)
GCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTC352050.11363380342426857Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGAACGTGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC346840.11195213287792448TruSeq Adapter, Index 6 (97% over 36bp)
TGCAGGACACATTGATCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC344080.11106126709905505Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCTGTGGGATTATGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC342200.11045444548156427Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGCAATAACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGT341430.11020590684035796Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCC337130.10881796377907586Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCGGGCGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT335340.1082401921326352TruSeq Adapter, Index 11 (100% over 37bp)
CACAGCCAAGGGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC331260.10692326011169778Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCT331100.10687161571871985TruSeq Adapter, Index 11 (100% over 43bp)
CGTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT324820.1048445732943358TruSeq Adapter, Index 11 (100% over 41bp)
TGGTTAGTACTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG324540.10475419560662441Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATTGAGCAATAACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCC323320.10436040711016763Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA321110.10364706893215986Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG318020.10264968659277345No Hit
AGTCTGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA317050.1023365924603447TruSeq Adapter, Index 11 (100% over 40bp)
CTGACTGGCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT312930.10100674934116279TruSeq Adapter, Index 11 (100% over 37bp)
CCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC311550.1005613164517281Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTGGCTTGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT310980.10037733330174418TruSeq Adapter, Index 11 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)