FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004974844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15491682
Sequences flagged as poor quality0
Sequence length150
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT7202724.6494112130625975No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG1069480.6903575738257473No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGC920730.5943383036135134No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG842320.5437240449423116No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT752750.48590592035132146No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC662780.4278295926807689No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT648070.418334174429865No Hit
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAG629990.4066633952336486No Hit
CAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA625650.40386189182039756TruSeq Adapter, Index 9 (100% over 40bp)
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCA623380.40239658934388145No Hit
TCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT544940.3517629654417125Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA509360.3287958015146451TruSeq Adapter, Index 9 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC502490.3243611636231624No Hit
ACAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC458980.2962751236437722TruSeq Adapter, Index 9 (100% over 37bp)
CCCACTAATAGGGAACGTGAGCAGATCGGAAGAGCACACGTCTGAACTCC453060.29245371806625003Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT434050.28018261671005124No Hit
AGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA411610.26569742394660567TruSeq Adapter, Index 7 (97% over 35bp)
AGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT399960.2581772592543534TruSeq Adapter, Index 9 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC397730.25673777708579354No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC384410.24813961453636862No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC374730.24189110001096073TruSeq Adapter, Index 9 (100% over 50bp)
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTAGATCGGAAGAGCACAC373460.2410713052333504No Hit
GTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC371370.23972219414263732Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG366020.23626872795349144Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG339780.2193306059342039TruSeq Adapter, Index 9 (100% over 39bp)
AGCAGCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC333220.21509607542938206Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA327940.21168779477915958No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG320470.20686585226833343No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT306370.19776419371376203TruSeq Adapter, Index 9 (100% over 48bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC292660.18891428316176384No Hit
ATCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAG281130.18147157939338027Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA278200.17958024183558635TruSeq Adapter, Index 9 (100% over 40bp)
TCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC269460.1739385045471499Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGA266690.1721504482211809No Hit
CTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC245920.15874325331490796TruSeq Adapter, Index 9 (100% over 42bp)
GAGCAATAACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC243340.15707784345173106Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT239960.15489602742942954TruSeq Adapter, Index 9 (100% over 36bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTA236940.1529465941787341TruSeq Adapter, Index 9 (100% over 47bp)
TTGGAGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA235070.1517394947817803Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGATTGTTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC232500.15008053999559248TruSeq Adapter, Index 9 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC231070.14915746398615723No Hit
TCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACG230240.14862169259606545No Hit
GGGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA226470.14618812857119065Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA221340.14287667407580404TruSeq Adapter, Index 9 (100% over 40bp)
CCGACTGTTTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT219100.14143073683025512TruSeq Adapter, Index 9 (100% over 36bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT214930.138738969725818TruSeq Adapter, Index 9 (100% over 43bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC209360.13514349184291286No Hit
GGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC208710.1347239118386241Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCC206670.13340707613285632Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA206330.13318760351522838TruSeq Adapter, Index 9 (100% over 40bp)
CGGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC204740.1321612462739682TruSeq Adapter, Index 9 (100% over 42bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG196810.12704237022164538Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTAGTTGGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT192050.12396975357485392TruSeq Adapter, Index 9 (100% over 36bp)
CGGCGACTCTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG191430.12356953880153235Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTA185470.11972231291605391TruSeq Adapter, Index 9 (100% over 47bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTA179560.1159073624155208TruSeq Adapter, Index 9 (100% over 47bp)
GTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGT179470.11584926672261928Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTGTGGGATTATGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA176630.11401602485772687Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCCTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA175580.11333824177387582TruSeq Adapter, Index 9 (100% over 40bp)
GGGGATGTAGCTCAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC175160.11306712854033539Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGGACGCTACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT175080.11301548792442292TruSeq Adapter, Index 9 (100% over 36bp)
AGCTCGTAGTTGGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA174430.11259590792013419Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCATCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTC172850.11157600575586306Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG169860.10964593773613478No Hit
CCGACTGTTTAATTAAAACAAAGCAAGATCGGAAGAGCACACGTCTGAAC169520.10942646511850683Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGACTGTTTAATTAAAACAAGATCGGAAGAGCACACGTCTGAACTCCAG168360.10867767618777611Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT167720.10826455126047643Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGCAATAACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGT167640.10821291064456397Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA166890.10772877987038465Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGAGATTGAGCAATAACAGGTCTGTAGATCGGAAGAGCACACGTCTGAAC164980.10649586016547462Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGT162910.10515965922873965Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT161960.10454642691477918TruSeq Adapter, Index 9 (100% over 43bp)
TGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA160620.10368144659824542Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC160570.10364917121330015TruSeq Adapter, Index 9 (100% over 42bp)
ATCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC155340.10027316594802295Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTGG1066200.0137.524081
CATTGGT1070700.0137.137562
ATTGGTG1092350.0134.60153
TGGTTCA1372600.0127.1930168
GGTGGTT1269000.0126.780256
GTGGTTC1393250.0125.375017
GGTTCAG1420350.0123.236339
TTGGTGG1207950.0122.7513354
TGGTGGT1401250.0121.047175
GTCCGAT275150.0120.731756
TCCGATG276750.0120.085787
CTGGTCC309150.0119.9365163
TGGTCCG356750.0117.39474
GGTCCGA365100.0114.966195
GCTGGTC332550.0111.7659152
CCGATGG298850.0111.374068
TCCCTGG198200.0109.4567261
CCCTGGT200050.0107.937532
GCATTGT128100.0105.4940261
GCATTTG125700.0103.598851
Produced by FastQC (version 0.11.5)