FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004974846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16219106
Sequences flagged as poor quality0
Sequence length150
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT10739846.621721320521612No Hit
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCA1533080.945230890037959No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG1415490.8727299766090683No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGC1341210.8269321379365792No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG1174860.7243679152229476No Hit
TGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1139280.7024308244856405Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1056450.6513614252228206No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC1032710.636724366928732No Hit
AGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC951150.5864379947945343TruSeq Adapter, Index 5 (97% over 35bp)
GCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTC794840.49006400229457775Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAG767210.47302853807108725No Hit
TCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT757780.46721440750186843Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC731540.45103595722230316No Hit
CCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCA714600.44059148512871177Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG660840.407445391873017No Hit
AGCAGCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC614220.37870151412784403Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC581890.358768232971657No Hit
CCGACTGTTTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG575810.35501956766297726TruSeq Adapter, Index 7 (97% over 36bp)
GTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC563100.3471831308088128Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA549020.338502011146607No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC528520.3258625968656966No Hit
TCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC510950.315029693991765Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC507860.3131245334977156TruSeq Adapter, Index 16 (100% over 38bp)
CAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCG501090.3089504440010442TruSeq Adapter, Index 16 (97% over 39bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT494700.3050106460861653No Hit
AGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG492730.30379602920160953TruSeq Adapter, Index 7 (97% over 36bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT490150.30220531267259737No Hit
TCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC443430.2733997792480054Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCACA420160.2590525026471866No Hit
TCTGCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAA407030.250957111939462Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAG406720.2507659793332629Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACG394290.24310217838147183No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC373660.23038261171731658No Hit
TACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGA358100.2207889879997085No Hit
ACAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT355700.21930925169365068TruSeq Adapter, Index 16 (100% over 37bp)
GCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC354630.21864953592386657TruSeq Adapter, Index 5 (97% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA354370.21848923115737698No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA353250.21779868754788334No Hit
AAGGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCG339610.20938885287512146TruSeq Adapter, Index 16 (97% over 39bp)
CCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACGTCTGA337530.2081064147432047Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC323260.19930814929010268Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC304650.18783402735021276No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC299240.1844984550936408No Hit
ACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCAC293040.18067580296965813No Hit
TTGGAGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA286970.17693330322892026Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT280940.17321546575995003Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCG277420.17104518584439857TruSeq Adapter, Index 16 (97% over 39bp)
CGTGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCG274630.16932499238860638TruSeq Adapter, Index 16 (97% over 39bp)
CACTACTCTGATCGTTTTTTCACTGACCAGATCGGAAGAGCACACGTCTG271750.167549308821337Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA271710.1675246465495694Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGTGGGATTATGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA259210.15981768662218496Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG256750.15830095690847573No Hit
TCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCC249000.1535226417534974Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG242480.14950269145537368TruSeq Adapter, Index 7 (97% over 36bp)
AGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCA237620.14650622543560662TruSeq Adapter, Index 16 (97% over 39bp)
ACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC233830.14416947518562367TruSeq Adapter, Index 16 (100% over 39bp)
CACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC233720.14410165393826269Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCAC232610.14341727589671094No Hit
CGGGCGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCG231670.14283771251017166TruSeq Adapter, Index 16 (97% over 39bp)
GACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCG229550.14153061210648726TruSeq Adapter, Index 16 (97% over 39bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCAC228820.141080525646728No Hit
ACAGCCAAGGGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC228750.14103736667113465Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTAGATCGGAAGAGCACAC227310.14014952488749996No Hit
GGGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA226660.13974876297127597Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGT225810.13922468969621385Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC218700.1348409708895176Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCC218580.1347669840742147Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGATTGTTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT216320.13337356571934358TruSeq Adapter, Index 16 (100% over 37bp)
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA215380.1327940023328043No Hit
CCACTACTCTGATCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC212540.13104298103730255Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCACACGT210360.1296988872259667No Hit
ACCATCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTC210070.12952008575565138Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCA203520.12548163875370197TruSeq Adapter, Index 16 (97% over 39bp)
TTCTGCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGA201280.12410055153471468Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCG198550.12241735148657393TruSeq Adapter, Index 16 (97% over 39bp)
CCCACTAATAGGGAACGTGAGCAGATCGGAAGAGCACACGTCTGAACTCC196740.12130138368908866Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGACTGGCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT192250.11853304368317219TruSeq Adapter, Index 16 (100% over 37bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCAT191500.1180706260875291TruSeq Adapter, Index 16 (97% over 39bp)
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC191260.11792265245692334No Hit
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCAT191080.11781167223396899TruSeq Adapter, Index 16 (97% over 39bp)
CACTACTCTGATCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC186830.11519130585865829TruSeq Adapter, Index 5 (97% over 35bp)
CAGATCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC186820.1151851402907164TruSeq Adapter, Index 5 (97% over 35bp)
TGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACGTCTGAACTC180410.11123301123995366Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCG178310.10993824197215309TruSeq Adapter, Index 16 (97% over 39bp)
CGGCGACTCTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC176450.10879144633495828Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAATGGATGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC175490.10819955181253517TruSeq Adapter, Index 5 (97% over 35bp)
CGTAGTTGGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG175350.10811323386134845TruSeq Adapter, Index 7 (97% over 36bp)
ATCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC173390.1069047825447346Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGC172300.10623273563906666TruSeq Adapter, Index 16 (97% over 39bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT169880.10474066819712503No Hit
CGGGCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGC169640.10459269456651926TruSeq Adapter, Index 16 (97% over 39bp)
CAGGTCTGTGATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC168600.10395147550056089TruSeq Adapter, Index 5 (97% over 35bp)
GAGCAATAACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC167290.10314378610017098Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGACTGTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT167240.10311295826046145TruSeq Adapter, Index 16 (100% over 37bp)
AGCTCGTAGTTGGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA163480.10079470471430423Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGGT1505850.0139.877792
GCATTGG1511000.0139.417791
ATTGGTG1530700.0137.681953
TGGTTCA1809900.0134.033288
GTGGTTC1836800.0132.15667
GGTGGTT1672350.0131.504886
GGTTCAG1855900.0131.099099
TCCGATG380300.0131.063787
GTCCGAT386050.0129.05576
TTGGTGG1657350.0128.17724
TGGTGGT1980400.0128.045245
CTGGTCC434100.0126.877993
CATTTGT176550.0125.677772
TCCCTGG332450.0125.432481
GGTCCGA459800.0125.329355
CCGATGG401150.0124.449098
TGGTCCG466250.0123.53384
CCCTGGT340900.0121.8043442
GCATTTG182200.0121.572081
GCTGGTC455550.0120.857562
Produced by FastQC (version 0.11.5)