FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004974847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20228943
Sequences flagged as poor quality0
Sequence length150
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGC4953262.4486005027548896No Hit
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCA3905101.930451828353068No Hit
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAG3520521.7403380888462636No Hit
TACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGA2048611.012712330051056No Hit
AATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAA1222540.6043518932254641No Hit
TCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAG1019150.5038078361286599No Hit
AGCAGCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC953730.47146803468673576Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGA623680.3083107209308959No Hit
ACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCAC608280.30069786641842833No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT539050.2664746249964716No Hit
ACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGAGATCGGAAGAGCAC503220.24876237972493173No Hit
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCACA486510.24050193823770227No Hit
CGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAGAGCACACG463660.22920624176952795No Hit
CCCACTAATAGGGAACGTGAGCAGATCGGAAGAGCACACGTCTGAACTCC438170.21660548452778774Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACAGCCTCTGGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTC437620.2163335968666282No Hit
CCGACTGTTTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT435350.21521144233784237TruSeq Adapter, Index 8 (100% over 36bp)
CGCGACCTCAGATCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCC432030.21357022954684285Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCG411730.20353510314404466No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA405890.20064815052373225No Hit
CGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAAT401290.19837418099403417No Hit
TTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAA392210.19388556287889092No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCAC387730.1916709142934458No Hit
TGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTAGATCGGAAGAG375110.1854323283228392No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG373170.18447330639074913Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACG372730.18425579626182148No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC368140.18198677014414447No Hit
AAAGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGT366610.1812304281049188Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTAGATCGGAAGAGCACAC359190.17756241638527528No Hit
GCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTC347920.17199119103751492Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTTGGATG343510.16981114633621736No Hit
CTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCACACGT321120.15874284682101283No Hit
CCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAGA315830.15612778186186No Hit
TGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGC306770.15164905057075895No Hit
AGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA306210.15137221949757831Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA303180.1498743656551902No Hit
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCA295270.14596412674651366No Hit
CAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGA295270.14596412674651366TruSeq Adapter, Index 25 (100% over 38bp)
AGCAGCCGACTTAGAACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG292620.1446541225609267Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTCTGATCTCGGAAGCTAAGCAGGGTCGAGATCGGAAGAGCACACGTCT283830.14030886339439486Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGTTAGGACCCGAAAGATGGTGAACTAGATCGGAAGAGCACACGTCTG279500.13816836598926596Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATA276500.1366853423829411TruSeq Adapter, Index 25 (100% over 40bp)
AGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC273280.13509356371215242TruSeq Adapter, Index 5 (100% over 35bp)
CCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGAACTCCAGT272480.1346980907504658Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGC271070.1340010696554931No Hit
CCCACTAATAGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTC264540.13077302160572601Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACGTCTGA262140.12958660272066613Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGACTGTTTAATTAAAACAAGATCGGAAGAGCACACGTCTGAACTCCAG260610.12883026068144046Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAAAAGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCC255630.12636844149494117Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTC251880.12451466198703512No Hit
TGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAG251470.12431198209417071No Hit
CGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTAGATCG249590.12338262063420714No Hit
GTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCG247070.12213688080489424No Hit
GGGGAATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGAA246060.12163759619076488Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACC244700.12096529215589762No Hit
ACAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTG243420.120332535417199TruSeq Adapter, Index 5 (97% over 37bp)
CGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGA238360.11783116893453109No Hit
AGCTACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTC228100.11275922820090008No Hit
GCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC220760.10913076377742524TruSeq Adapter, Index 5 (100% over 35bp)
GATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCA219530.10852272409883204No Hit
AAAGAACTTTGAAGAGAGAGTAGATCGGAAGAGCACACGTCTGAACTCCA219010.10826566667373573Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGACTGTTTAATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC218400.1079641185404497TruSeq Adapter, Index 5 (100% over 35bp)
GACGAAAAATAACAATACAGGACTCTAGATCGGAAGAGCACACGTCTGAA211700.10465203248632418Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAATGGATGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC209800.10371278420231844TruSeq Adapter, Index 5 (100% over 35bp)
CCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC207430.1025411955533218No Hit
AGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGATCGGAA206180.10192326905068642No Hit
AGGGGAATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGA205990.10182934422228586Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTCA178700.0113.414234
TGGTTCA134250.0111.9236458
ACCTCAG182400.0110.9951865
CATTGGT115250.0106.8046652
GCGACCT191500.0106.013542
CCTCAGA191900.0105.462866
GCATTGG118650.0104.000891
TCCCTGG108600.099.1227651
CGACCTC214350.095.021783
CCCTGGT113850.094.698222
GTGGTTC162600.092.3650057
CTGGTCC88150.086.085893
TGTGAAC132400.084.960611
CTCAGAT236950.084.7737057
GGTTCAG178300.084.716469
CAGATCA235850.084.2532969
TCCGATG68600.082.282157
AATACCA318850.081.502511
GGTCCGA103150.081.454545
AGCGATT118450.081.365021
Produced by FastQC (version 0.11.5)