FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004974848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13674123
Sequences flagged as poor quality0
Sequence length150
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGC2511661.8367978699621172No Hit
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAG1787231.3070161793922725No Hit
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCA1706921.2482848077350188No Hit
TACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGA928080.6787126311500927No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT562650.4114706295972327No Hit
AATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAA554050.40518137799404025No Hit
AGCAGCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC552820.40428186875311856Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG404620.29590197484694264TruSeq Adapter, Index 2 (97% over 35bp)
ACAGCCTCTGGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTC381940.2793159020143376No Hit
TCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAG363020.2654795484873143No Hit
CCCACTAATAGGGAACGTGAGCAGATCGGAAGAGCACACGTCTGAACTCC355690.26011905845808175Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC319500.233653010141857No Hit
CAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCG307270.22470910931545665TruSeq Adapter, Index 14 (100% over 40bp)
ACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCAC296420.2167744139788709No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCG287710.21040471845982373TruSeq Adapter, Index 14 (97% over 40bp)
ACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGAGATCGGAAGAGCAC272720.19944240665379417No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTAGATCGGAAGAGCACAC269490.19708028076096726No Hit
AAAGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGT265270.19399415962544728Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTAGATCGGAAGAG252470.18463341305325395No Hit
CGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAGAGCACACG247390.1809183667574147No Hit
TCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGA246760.1804576425120646No Hit
AGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA243850.1783295352835425Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCC239520.1751629702321677Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT237990.17404406849346024TruSeq Adapter, Index 8 (97% over 36bp)
GGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTCTGAACTCCA227900.16666516748459845Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCACA226350.16553163957937192No Hit
TCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT225410.16484420975297648Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG224990.1645370602560764Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCC222460.16268685019141632Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGTAATTCTAGAGCTAATACATGCCGACGAGATCGGAAGAGCACACG219030.16017846263339885No Hit
TCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACG214670.1569899583322455No Hit
CAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTC213020.15578329959442372TruSeq Adapter, Index 14 (100% over 38bp)
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCAC211070.15435724835881612No Hit
CCGACTGTTTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT210810.15416710819406845TruSeq Adapter, Index 8 (97% over 36bp)
TTGGAGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA208980.1528288139575752Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGC196390.1436216421338319No Hit
GTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGT196180.14346806738538187Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCA195390.1428903338078793No Hit
ACAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTT193940.14182993673524802TruSeq Adapter, Index 8 (97% over 37bp)
GACGAAAAATAACAATACAGGACTCTAGATCGGAAGAGCACACGTCTGAA187280.1369594232844037Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTC177970.13015094276978495No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG175100.12805208787430097No Hit
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAAGATCGGAAGAGCA170910.12498790598855956No Hit
CCCACTAATAGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTC170120.124410172411057Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTC169040.12362035941902817Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGCAATAACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC162480.1188229768007791Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCAGCCGACTTAGAACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG157100.1148885380071541Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCC153170.11201449628616036TruSeq Adapter, Index 14 (100% over 39bp)
AAAGAACTTTGAAGAGAGAGTAGATCGGAAGAGCACACGTCTGAACTCCA152210.11131244029324587Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA150680.1101935385545384Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTAGATCGGAAGAGC148150.1083433284898783No Hit
TCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA148090.10829944999032114Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAGA147400.10779484724541383No Hit
CCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC145860.10666863242344683No Hit
TGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGA145290.10625178667765384No Hit
CCGACTGTTTAATTAAAACAAGATCGGAAGAGCACACGTCTGAACTCCAG143920.10524989427109878Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAA142850.10446739436232948Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAATGGATGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG142560.10425531494780323TruSeq Adapter, Index 2 (97% over 35bp)
CTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCACACGT142510.1042187495315056No Hit
AAGGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCG141860.10374339911963641TruSeq Adapter, Index 14 (100% over 40bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT141020.10312910012583623No Hit
AGCTACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTC140730.10291702071130997No Hit
CGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGA139770.10221496471839546No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTGG123300.0113.503721
CATTGGT123600.0113.232972
TGGTTCA147300.0111.588128
GTCCGAT85850.0105.1651846
TCCGATG86400.0104.079087
GACCTCA101150.0103.635914
CTGGTCC108300.099.6524353
ATTGGTG140700.099.373343
TCCCTGG98900.099.281131
ACCTCAG106450.098.543655
GGTCCGA122850.097.812865
GTGGTTC169650.096.844867
CCCTGGT102100.096.208242
CCTCAGA110150.095.233526
GCGACCT112350.094.102072
TGGTCCG129300.093.156294
CCGATGG99000.091.4144448
GGTTCAG187550.087.985749
CAGATCA117600.087.302569
GCTGGTC126650.085.411752
Produced by FastQC (version 0.11.5)