FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004974850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21499169
Sequences flagged as poor quality0
Sequence length150
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT9250254.302608161273582No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGC2629361.223005410116084No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1857130.863814782794628No Hit
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAG1835140.8535864804821061No Hit
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCA1677960.7804766779590411No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1573260.7317771212459422No Hit
AGCAGCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1107190.5149919980628088Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG976970.45442221510980263No Hit
AGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT956590.4449427789511306TruSeq Adapter, Index 1 (97% over 35bp)
TACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGA803520.3737446782245398No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC701940.32649634039343567No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC637730.2966300697482772TruSeq Adapter, Index 12 (100% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG637430.29649052947116233No Hit
CCCACTAATAGGGAACGTGAGCAGATCGGAAGAGCACACGTCTGAACTCC579780.26967553955224965Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG510150.23728824123388212Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT488530.22723203859646857TruSeq Adapter, Index 12 (100% over 43bp)
CGCGACCTCAGATCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCC472700.21986896330737246Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT467440.21742235711529131Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT462040.21491063212722314TruSeq Adapter, Index 12 (100% over 36bp)
GGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC437370.20343577000580815Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC429460.19975655803254536No Hit
CCGACTGTTTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT419440.1950959127769078TruSeq Adapter, Index 12 (100% over 36bp)
AATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAA417610.19424471708650692No Hit
CAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA417300.19410052546682152TruSeq Adapter, Index 12 (100% over 40bp)
CCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT385200.17916971581552757TruSeq Adapter, Index 12 (100% over 36bp)
CAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT379930.17671845828087587TruSeq Adapter, Index 12 (100% over 38bp)
TCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC379810.17666264217002992Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG373850.17389044199801398TruSeq Adapter, Index 12 (100% over 37bp)
TCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCACACG373430.17369508561005312No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT370480.17232293955175665No Hit
ACAGCCAAGGGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC368140.17123452539026043Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCC367310.17084846395690922Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTC365890.1701879733118987Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA365420.16996936021108539Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGATGTAGCTCAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC365110.16982516859139996Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTCTGAACTCCA364050.16933212627892735Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATC350440.16300164904048153TruSeq Adapter, Index 12 (100% over 42bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA345150.16054108882068882TruSeq Adapter, Index 12 (100% over 40bp)
GGGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA344500.16023875155360656Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGAGATCGGAAGAGCAC339270.15780609938923687No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTAGATCGGAAGAGCACAC338010.1572200302253543No Hit
TCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAG336420.15648046675664534No Hit
TCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC331030.1539733931111477Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGAGATCGGAAGAGCAC330500.15372687195491136No Hit
GTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGT330000.15349430482638654Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAGAACTTTGAAGAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGT324750.15105234997687583Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACAGCCTCTGGCATGTTGGAACAATGTAGGAGATCGGAAGAGCACACGTC320330.14899645656071636No Hit
CCGACTGTTTAATTAAAACAAGATCGGAAGAGCACACGTCTGAACTCCAG319120.14843364410968626Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC314700.14637775069352682Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGCAATAACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC312990.1455823711139719Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT312970.14557306842883092TruSeq Adapter, Index 12 (100% over 48bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA303680.1412519711808396No Hit
CGGCGACTCTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC303560.14119615506999364Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAATGGATGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT301870.1404100781755797TruSeq Adapter, Index 1 (97% over 35bp)
AGCAGCCGACTTAGAACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG298740.1389542079510143Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA297810.1385216330919581TruSeq Adapter, Index 12 (100% over 39bp)
GCCGACTTAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT287940.13393075797487802TruSeq Adapter, Index 1 (97% over 35bp)
CGAACGAGACTCTGGCATGCTAACTAGTTACGAGATCGGAAGAGCACACG285660.1328702518688048No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC282740.13151205983821979No Hit
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTA282550.13142368432938037TruSeq Adapter, Index 12 (100% over 47bp)
TTGGAGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA279390.12995386007710344Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA278430.12950733119033578TruSeq Adapter, Index 12 (100% over 40bp)
CGGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATC277680.12915848049754852TruSeq Adapter, Index 12 (100% over 42bp)
GACTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA272000.12651651791750648TruSeq Adapter, Index 12 (100% over 40bp)
CGTAGTTGGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT264700.12312103784104399TruSeq Adapter, Index 12 (100% over 36bp)
CCCACTAATAGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTC262440.12206983442011178Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGACTGGCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG262070.1218977347450034TruSeq Adapter, Index 12 (100% over 37bp)
CCTGTCTGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG253740.11802316638377976TruSeq Adapter, Index 12 (100% over 37bp)
ATCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAG251900.11716731935080839Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTCGTAGTTGGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA249840.11620914278128609Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCATCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTC248280.11548353334028863Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGAGGTGTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG245400.11414394667998563TruSeq Adapter, Index 12 (100% over 37bp)
GATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAGATCGGAAGAGCA244520.11373462853378194No Hit
ATTTTGTTGGTTTTCGGAACTAGATCGGAAGAGCACACGTCTGAACTCCA242330.11271598451084318Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC242110.11261365497429227No Hit
CGTAGTTGGATCTTGGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCA241750.11244620664175439Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATC240350.11179501868188488TruSeq Adapter, Index 12 (100% over 42bp)
CAGCCAAGGGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT239770.11152524081279606TruSeq Adapter, Index 1 (97% over 35bp)
GTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA232930.10834372249457641Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGACTCTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG231740.10779021272868733TruSeq Adapter, Index 12 (100% over 37bp)
ACCACTACTCTGATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA229590.10679017407603057Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCGTCTATGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC228730.10639015861496787Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC228190.10613898611616104No Hit
AGCCTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA227570.10585060287679027TruSeq Adapter, Index 12 (100% over 40bp)
AGCCGGGCGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG227500.1058180434787968TruSeq Adapter, Index 12 (100% over 37bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT226840.10551105486914401TruSeq Adapter, Index 12 (100% over 43bp)
ACTGGCTTGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG222690.10358074770238793TruSeq Adapter, Index 12 (100% over 37bp)
CAGGTCTGTGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT217010.1009387851223459TruSeq Adapter, Index 12 (100% over 36bp)
ACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAA215790.10037132132874531Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)