FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004974851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15582756
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT176113611.301826198138507No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG3261272.0928711198455523No Hit
TGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1696221.088523750227495Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG1466970.9414060003249746No Hit
AGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1453640.93285167270796TruSeq Adapter, Index 1 (100% over 35bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1380700.8860435214412649No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1327170.8516914466221508No Hit
GTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1313560.8429574332037285Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT1204960.7732650116577581Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTT957200.6142687468121814TruSeq Adapter, Index 27 (100% over 40bp)
TCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA881850.5659140141833704Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC839630.5388199622711156No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA817540.5246440360100614No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC730340.46868474357167633No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT727860.46709324075920844No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC727360.46677237325669474No Hit
TCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC720690.4624920007731624Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGGATTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCT718660.46118927871295684TruSeq Adapter, Index 27 (100% over 39bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC710010.45563827091947023No Hit
GGCATGTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCC695830.44653846854818235TruSeq Adapter, Index 27 (100% over 38bp)
CAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTT689680.4425917982672642TruSeq Adapter, Index 27 (100% over 40bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC685280.4397681642451438No Hit
AGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT634210.406994757538397TruSeq Adapter, Index 8 (97% over 36bp)
AGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTA633230.4063658572334701TruSeq Adapter, Index 27 (97% over 41bp)
TTGGATTGTTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTC623400.4000576021340512TruSeq Adapter, Index 13 (97% over 37bp)
GGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC603460.3872614061338059Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCTGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAG588300.37753270345759116Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGCGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTT573240.3678681742818793TruSeq Adapter, Index 27 (100% over 40bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC568870.36506379230990976No Hit
CGTAGTTGGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT540910.34712088156934495TruSeq Adapter, Index 8 (97% over 36bp)
GGGTTTCGTACGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA522640.33539638302749525Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGTGGGATTATGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA519590.33343909126216187Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG462150.29657783257339076No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC451060.2894609913676374No Hit
GTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA438090.28113768835243264Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTA433350.278095864428603TruSeq Adapter, Index 27 (97% over 41bp)
AGCCTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTT430220.2760872338628674TruSeq Adapter, Index 27 (100% over 40bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG421540.27051697401922997No Hit
ACAGCCAAGGGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA405490.260217127188541Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTCGTAGTTGGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA403760.25910692562984367Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCA402350.25820207927275507Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCT398420.2556800607029976TruSeq Adapter, Index 27 (100% over 39bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTT396560.2544864335936467TruSeq Adapter, Index 27 (100% over 40bp)
GGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT394090.25290134813122916TruSeq Adapter, Index 1 (100% over 35bp)
GTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTC391270.25109165541705203TruSeq Adapter, Index 13 (97% over 37bp)
ACAGCAGTTGAACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT379170.2433266618562211TruSeq Adapter, Index 1 (100% over 35bp)
GTGGGATTATGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT364240.2337455582311627TruSeq Adapter, Index 1 (100% over 35bp)
CAGGTCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCC358490.23005558195225542TruSeq Adapter, Index 27 (100% over 38bp)
GCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTC344290.22094294488086705Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT336930.2162197752438657Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTA334800.21485287968315744TruSeq Adapter, Index 27 (97% over 41bp)
ACAGGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCT332040.21308169106928196TruSeq Adapter, Index 27 (100% over 39bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA323140.20737024952453853No Hit
CTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTT293290.18821445962447209TruSeq Adapter, Index 27 (100% over 41bp)
AGACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTT289210.18559618080396048TruSeq Adapter, Index 27 (100% over 40bp)
CGGGCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTT287180.18429345874375497TruSeq Adapter, Index 27 (100% over 41bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT285570.18326026538566093No Hit
GGGGATGTAGCTCAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC284690.18269553858123685Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAGCCAAGGGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT280750.18016710266142907TruSeq Adapter, Index 1 (100% over 35bp)
CGGGGAGGTAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTC273130.17527708192312066TruSeq Adapter, Index 13 (97% over 37bp)
AAGGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTT260310.16705003915867TruSeq Adapter, Index 27 (100% over 40bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA258000.16556763129705684No Hit
CGTGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTT252990.1623525389218698TruSeq Adapter, Index 27 (100% over 40bp)
CGTGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTT237880.1526559229959065TruSeq Adapter, Index 27 (100% over 41bp)
CAGGGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTT236850.15199493594072833TruSeq Adapter, Index 27 (100% over 41bp)
TCTGTGGGATTATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT236340.15166765108816438TruSeq Adapter, Index 1 (100% over 35bp)
ATCTGTGGGATTATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC219660.14096351120430814Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGAGGGCAAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA219080.1405913049013923Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CATCTGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTT218140.13998807399666657TruSeq Adapter, Index 27 (100% over 40bp)
GCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC214410.13759440242791457Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGATTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTT210030.13478360310589477TruSeq Adapter, Index 27 (100% over 40bp)
CAGGTGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCT206730.13266587758930448TruSeq Adapter, Index 27 (100% over 39bp)
CACAGCCAAGGGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC201790.12949570666446936Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGCTCGTAGTTGGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC200050.12837908775572177Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT199430.12798121205260482TruSeq Adapter, Index 8 (97% over 36bp)
TCTGCCCAGTGCTCTGAATGTCAAAGAGATCGGAAGAGCACACGTCTGAA195140.12522816888103747Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGGATTATGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA194010.12450300832535657Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGGCATGTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT191950.12318103421500022TruSeq Adapter, Index 8 (97% over 36bp)
CCCGTCTATGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA187490.12031889609257823Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTT186030.1193819629852383TruSeq Adapter, Index 27 (100% over 41bp)
CGCGGACGCTACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT185590.11909959958302627TruSeq Adapter, Index 8 (97% over 36bp)
AGTCTGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTT185420.11899050463217162TruSeq Adapter, Index 27 (100% over 40bp)
AGCAGTTGAACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTC185360.11895200053186998TruSeq Adapter, Index 13 (97% over 37bp)
TGGTTAGTACTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA170680.10953133065806843Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCCTGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTT170240.10924896725585641TruSeq Adapter, Index 27 (100% over 40bp)
CCTCGGATAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCC168170.1079205757954498TruSeq Adapter, Index 27 (100% over 38bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCAC167020.10718258053966834No Hit
AGCCGGGCGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTC163320.10480816102106713TruSeq Adapter, Index 13 (97% over 37bp)
GGGTTTCGTACGTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA161020.10333217050950423Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCTGTGGGATTATGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC160910.10326157965895122Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGGAACGTGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT160340.10289579070608562TruSeq Adapter, Index 8 (97% over 36bp)
GCAGTTGAACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCC160010.1026840181544266TruSeq Adapter, Index 27 (100% over 38bp)
TAGGGAACGTGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA158550.10174708504708666Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTAT157160.10085507339009864TruSeq Adapter, Index 27 (97% over 43bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTGG2311100.0141.021121
CATTGGT2313600.0140.983522
ATTGGTG2357950.0138.47563
TGGTTCA2770950.0135.766928
GTCCGAT657200.0135.272816
TCCGATG659050.0134.980487
GTGGTTC2799300.0134.489677
GGTTCAG2807000.0134.279779
GGTGGTT2619200.0133.591576
CTGGTCC718950.0133.117693
GGTCCGA753750.0133.056235
CCGATGG670650.0132.871228
TTGGTGG2494050.0131.328954
CGATGGT677900.0131.322749
TGGTCCG764450.0131.099674
TGGTGGT2798350.0130.423975
GCTGGTC738800.0129.520432
TCCCTGG294900.0126.904041
CCCTGGT301800.0123.668512
GCATTTG208100.0123.24711
Produced by FastQC (version 0.11.5)