FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004974856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23960716
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC37150.022.4387956
CGTTGGA139600.021.7357981
CCGATCT44450.018.90257
CTTCCGA54900.018.7706189
ACGGGAT98700.018.3786689
AAACGGG159450.018.1552967
AACGGGA132250.018.0928738
TTCCGAT48750.017.4365165
GAAACGG168200.016.842046
TCTTCCG67300.016.6757568
GGAAACG173550.016.3263265
CGTGTGC79000.013.5508671
AACGGGT38350.011.5952128
AAGCGGC42200.010.4150623
CAAAGCG99400.010.2705411
GTTGGAA344050.09.6222782
CGATCTA17053.6379788E-129.5215358
ACGGGTT41450.09.0250449
ACGGGAA70000.08.9740829
AAAGCGG60200.08.4784932
Produced by FastQC (version 0.11.5)