FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004974861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22846324
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG255180.11169411761822164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC33400.027.280616
CCGATCT40100.022.7094677
TTCCGAT43150.020.840565
CTTCCGA50350.018.4229134
CGTTGGA128900.017.2633061
TCTTCCG61050.015.0780133
AACGGGA113450.015.0535328
ACGGGAT87650.014.6536589
AAACGGG142250.014.4411687
GAAACGG149900.013.5706836
GGAAACG158800.012.7972865
AACGGGT38300.09.5814698
CGATCTA18051.4551915E-118.9924538
AAGCGGC36600.08.899453
CGATCGG8000.08.538149140-144
GTTGGAA315800.08.4306722
CAAAGCG78850.08.1787491
CGCTCTT49850.07.2502371
GATTTCG76400.06.9511135
CGTGTGC69400.06.8416761
Produced by FastQC (version 0.11.5)