FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004974862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004974862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2953200
Sequences flagged as poor quality0
Sequence length1-22797
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCTA75250.059.204211
GCACCTC117650.055.6064641
GTTGTAC22900.043.9146421
GCACCTT104400.043.7092971
GCACCTG123500.043.4286731
TACTTCG25000.039.799345
CGCTTCG5500.039.329417
CACCTAC40000.036.226082
ACTTCGT31100.031.2980676
CACCTAA45400.030.7263132
CACCTAG42450.030.0594392
CACCTTA54750.029.824242
TCGTTCA46450.029.8044959
CTTCGTT53700.028.8012877
CGTTCGG6904.456604E-428.354805470-479
GTACTTC37800.027.7519344
GCTTCGT20550.026.8406946
TTCGTTC56900.026.2311278
CACCTCA99250.025.822272
GTAGCGC5457.2759576E-1225.7947163
Produced by FastQC (version 0.11.5)