FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004975267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004975267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10082
Sequences flagged as poor quality0
Sequence length58-31520
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC5034.989089466375719No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGT800.7934933544931562No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTC720.7141440190438405No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCT690.6843880182503471No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTC590.5852013489387026No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGTTCATGCTC590.5852013489387026No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCACAGAGTTCATGCT570.5653640150763737No Hit
AGTAACTGAAAATGCTTCTGAGTGGCTAGGATAACAGATGTGCACCACCA440.4364213449712359No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCC430.42650267804007147No Hit
AGTAACTGAAAATACTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC370.3669906764530847No Hit
AGTAACTGAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT360.35707200952192025No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTC340.33723467565959137No Hit
AGTAACTGAAAATCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT330.3273160087284269No Hit
TTGAAGAGAGGTGACAAAACAAAGGGGAGTATCCAACAATCTTCCCAAGG320.3173973417972624No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACGAGTTCATGCT290.2876413410037691No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGGATTCATGC260.2578853402102757No Hit
TTGAAGAGAGGTGACAAAACAAGGGGAGTATCCAACAATCTTCCCAAGGT260.2578853402102757No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCC230.2281293394167824No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAAGG220.21821067248561796No Hit
TTGAAGAGAGGTGACAAAACAAAGGGGAGTATCAACAATCTTCCCAAGGT220.21821067248561796No Hit
AGTAACTGAAAATAACTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATG210.20829200555445349No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCCTGAGTTCATGCT210.20829200555445349No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGGTTCATGCT200.19837333862328904No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCT170.16861733782979568No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGTTCATGCTC170.16861733782979568No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTCCT160.1586986708986312No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAAGGTC160.1586986708986312No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTCC150.14878000396746677No Hit
AGTAACTGAAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATG150.14878000396746677No Hit
TTGAAGAGAGGTGACAAAACAAGGGGAGTATCAACAATCTTCCCAAGGTC140.13886133703630232No Hit
AGTAACTGAAAATGCTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT140.13886133703630232No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTCT130.12894267010513785No Hit
TTGAAGAGGTGACAAAACAAGGGGAGTATCCAACAATCTTCCCAAGGTCC120.11902400317397342No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAGTTCATGCTCT120.11902400317397342No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAAGGT110.10910533624280898No Hit
TTGAAGAGAGGTGACAAAACAAAGGAAATGCAGAGGAGACCTTGACATAA110.10910533624280898No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCT110.10910533624280898No Hit
TTGAAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCCTG110.10910533624280898No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATAC110.10910533624280898No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)